© 2016

SiRNA Delivery Methods

Methods and Protocols

  • Kato Shum
  • John Rossi
  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1364)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Suvarna Dash-Wagh, Ülo Langel, Mats Ulfendahl
    Pages 27-35
  3. Min Tang, Haiqing Dong, Xiaojun Cai, Haiyan Zhu, Tianbin Ren, Yongyong Li
    Pages 49-61
  4. Kristen L. Kozielski, Jordan J. Green
    Pages 79-87
  5. Hyun Jin Kim, Meng Zheng, Kanjiro Miyata, Kazunori Kataoka
    Pages 89-103
  6. Jessica Wahlgren, Luisa Statello, Gabriel Skogberg, Esbjörn Telemo, Hadi Valadi
    Pages 105-125
  7. Xiaoxuan Liu, Ling Peng
    Pages 127-142
  8. Héloïse Ragelle, Kevin Vanvarenberg, Gaëlle Vandermeulen, Véronique Préat
    Pages 143-150
  9. King Sun Siu, Yujuan Zhang, Xiufen Zheng, James Koropatnick, Wei-Ping Min
    Pages 151-163
  10. Kun Xu, Zhongtian Liu, Long Zhang, Tingting Zhang, Zhiying Zhang
    Pages 165-181
  11. Dewan Md Sakib Hossain, Dayson Moreira, Qifang Zhang, Sergey Nechaev, Piotr Swiderski, Marcin Kortylewski
    Pages 183-196
  12. Carla L. Esposito, Silvia Catuogno, Vittorio de Franciscis
    Pages 197-208
  13. David D. Dickey, Gregory S. Thomas, Justin P. Dassie, Paloma H. Giangrande
    Pages 209-217
  14. Irmgard S. Thorey, Michael Grote, Klaus Mayer, Ulrich Brinkmann
    Pages 219-234

About this book


This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including peptide-based nanoparticles, liposomes, exosomes, polymers, aptamers, and viral vehicles. Written in the highly successful Methods in Molecular Biology series format, each proven protocol includes brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.


RNA interference adenoviruses antibodies dendrimers lentiviruses lipid-based nanoparticles peptides small interfering RNAs vector-expressed short hairpin RNAs viral vectors

Editors and affiliations

  • Kato Shum
    • 1
  • John Rossi
    • 2
  1. 1.Beckman Research Institute of City of HopeDuarteUSA
  2. 2.Beckman Research Institute of City of HopeDuarteUSA

Bibliographic information

  • Book Title SiRNA Delivery Methods
  • Book Subtitle Methods and Protocols
  • Editors Kato Shum
    John Rossi
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3111-8
  • Softcover ISBN 978-1-4939-4910-6
  • eBook ISBN 978-1-4939-3112-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIII, 313
  • Number of Illustrations 29 b/w illustrations, 35 illustrations in colour
  • Topics Human Genetics
  • Buy this book on publisher's site