Book Volume 110 2016

Receptor and Ion Channel Detection in the Brain

Methods and Protocols

Editors:

ISBN: 978-1-4939-3063-0 (Print) 978-1-4939-3064-7 (Online)

Table of contents (29 protocols)

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  1. Front Matter

    Pages i-xviii

  2. Molecular Techniques

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-18

      Production of High-Quality Antibodies for the Study of Receptors and Ion Channels

    3. No Access

      Protocol

      Pages 19-29

      Co-immunoprecipitation from Brain

    4. No Access

      Protocol

      Pages 31-37

      Subsynaptic Membrane Fractionation

    5. No Access

      Protocol

      Pages 39-48

      Investigation of Neurotransmitter Receptors in Brain Slices Using Cell Surface Biotinylation

    6. No Access

      Protocol

      Pages 49-58

      Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells

    7. No Access

      Protocol

      Pages 59-76

      Radioligand Binding Detection of Receptors in Brain Membranes

    8. No Access

      Protocol

      Pages 77-97

      Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain

    9. No Access

      Protocol

      Pages 99-107

      Fluorescent Ligands and TR-FRET to Study Receptor–Receptor Interactions in the Brain

    10. No Access

      Protocol

      Pages 109-124

      In Situ Proximity Ligation Assay to Study and Understand the Distribution and Balance of GPCR Homo- and Heteroreceptor Complexes in the Brain

  3. Neuroanatomical Techniques

    1. Front Matter

      Pages 125-125

    2. No Access

      Protocol

      Pages 127-142

      Fluorescent In Situ Hybridization for Sensitive and Specific Labeling

    3. No Access

      Protocol

      Pages 143-156

      Autoradiographic Visualization of G Protein-Coupled Receptors in Brain

    4. No Access

      Protocol

      Pages 157-170

      Analysis of the Expression Profile and Regional Distribution of Neurotransmitter Receptors and Ion Channels in the Central Nervous System Using Histoblots

    5. No Access

      Protocol

      Pages 171-178

      Immunohistochemistry for Ion Channels and Their Interacting Molecules: Tips for Improving Antibody Accessibility

    6. No Access

      Protocol

      Pages 179-190

      Localization of GFP-Tagged Proteins at the Electron Microscope

    7. No Access

      Protocol

      Pages 191-210

      Pre-embedding Methods for the Localization of Receptors and Ion Channels

    8. No Access

      Protocol

      Pages 211-232

      Post-embedding Immunohistochemistry in the Localisation of Receptors and Ion Channels

    9. No Access

      Protocol

      Pages 233-245

      High-Resolution Localization of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)

    10. No Access

      Protocol

      Pages 247-266

      Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies

    11. No Access

      Protocol

      Pages 267-275

      Analysis of Synaptic Connections at the Electron Microscopic Level Using Viral Vectors

    12. No Access

      Protocol

      Pages 277-309

      Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells

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