Mitochondrial DNA

Methods and Protocols

  • Matthew McKenzie

Part of the Methods in Molecular Biology book series (MIMB, volume 1351)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Detection of Mitochondrial DNA Mutations and Mitochondrial DNA Damage

    1. Front Matter
      Pages 1-1
    2. Meagan E. Palculict, Victor Wei Zhang, Lee-Jun Wong, Jing Wang
      Pages 3-17
    3. William C. Copeland, Rajesh Kasiviswanathan, Matthew J. Longley
      Pages 19-26
    4. Wei Wang, Katja Scheffler, Ying Esbensen, Lars Eide
      Pages 27-32
    5. Yevgenya Kraytsberg, Xinhong Guo, Saisai Tao, Alexandra Kuznetsov, Catherine MacLean, Daniel Ehrlich et al.
      Pages 33-46
  3. Visualization of Mitochondrial DNA and Mitochondrial DNA Nucleoids

    1. Front Matter
      Pages 47-47
    2. Anna Ligasová, Karel Koberna
      Pages 81-92
  4. Analysis of Mitochondrial DNA Replication, Mitochondrial DNA-Encoded Protein Translation and Mitochondrial DNA Copy Number

    1. Front Matter
      Pages 93-93
    2. Luke E. Formosa, Annette Hofer, Christin Tischner, Tina Wenz, Michael T. Ryan
      Pages 115-129
    3. Xin Sun, William Lee, Vijesh Vaghjiani, Justin C. St. John
      Pages 131-141
  5. Modification of Mitochondrial DNA

    1. Front Matter
      Pages 143-143
    2. Payam A. Gammage, Lindsey Van Haute, Michal Minczuk
      Pages 145-162
    3. Jaroslav Zelenka, Petr Ježek
      Pages 175-181
  6. Purification of Proteins Involved in Mitochondrial DNA Replication and Transcription

    1. Front Matter
      Pages 183-183
    2. Fernando A. Rosado-Ruiz, Minyoung So, Laurie S. Kaguni
      Pages 185-198
    3. John P. Gajewski, Jamie J. Arnold, Tiina S. Salminen, Laurie S. Kaguni, Craig E. Cameron
      Pages 199-210
    4. Grzegorz L. Ciesielski, Fernando A. Rosado-Ruiz, Laurie S. Kaguni
      Pages 211-222
  7. Yevgenya Kraytsberg, Xinhong Guo, Saisai Tao, Alexandra Kuznetsov, Catherine MacLean, Daniel Ehrlich et al.
    Pages E1-E1
  8. Back Matter
    Pages 233-234

About this book


This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and  mitochondrial DNA copy number,  the latest technologies for modifying the mitochondrial genome, and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.


mitochondria mitochondrial DNA analysis mitochondrial DNA copy number mitochondrial DNA damage mitochondrial DNA replication mitochondrial DNA-encoded protein translation mitochondrial genome mutations next-generation massive parallel sequencing replication and transcription

Editors and affiliations

  • Matthew McKenzie
    • 1
  1. 1.Hudson Institute of Medical ResearchClayton, MelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3039-5
  • Online ISBN 978-1-4939-3040-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site