Single Cell Protein Analysis

Methods and Protocols

  • Anup K. Singh
  • Aarthi Chandrasekaran

Part of the Methods in Molecular Biology book series (MIMB, volume 1346)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Elly Sinkala, Amy E. Herr
    Pages 1-9
  3. Sylvia Janetzki, Rachel Rabin
    Pages 27-46
  4. Nalin Leelatian, Kirsten E. Diggins, Jonathan M. Irish
    Pages 99-113
  5. Mei Ling Leong, Evan W. Newell
    Pages 115-131
  6. Catherine M. Rawlins, Joseph P. Salisbury, Daniel R. Feldman, Sinan Isim, Nathalie Y. R. Agar, Ed Luther et al.
    Pages 133-149
  7. Alexander Dünkler, Reinhild Rösler, Hans A. Kestler, Daniel Moreno-Andrés, Nils Johnsson
    Pages 151-168
  8. Matthias Blazek, Günter Roth, Roland Zengerle, Matthias Meier
    Pages 169-184
  9. Stephen Anthony, Amanda Carroll-Portillo, Jerilyn Timlin
    Pages 185-207
  10. Cinzia Tesauro, Rikke Frøhlich, Magnus Stougaard, Yi-Ping Ho, Birgitta R. Knudsen
    Pages 209-219
  11. Livia Shehaj, Lorena Lazo de la Vega, Michelle L. Kovarik
    Pages 221-238
  12. Back Matter
    Pages 253-254

About this book


This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation, and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.


Cell Signaling Flow Cytometry Gel Electrophoresis Heterogeneous Immunoassays Mass Spectrometry Microfluids Proteins Proteome Translocation

Editors and affiliations

  • Anup K. Singh
    • 1
  • Aarthi Chandrasekaran
    • 2
  1. 1.Sandia National Laboratories LivermoreUSA
  2. 2.Sandia National LaboratoriesLivermoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2986-3
  • Online ISBN 978-1-4939-2987-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site