Methods and Protocols

  • Albert C. Shaw

Part of the Methods in Molecular Biology book series (MIMB, volume 1343)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa, Rafael Solana
    Pages 9-18
  3. Xinyuan Hu, Erin M. DeBiasi, Erica L. Herzog
    Pages 19-33
  4. Aleah L. Brubaker, Stewart R. Carter, Elizabeth J. Kovacs
    Pages 35-51
  5. Samit R. Joshi, Subhasis Mohanty, Albert C. Shaw
    Pages 53-64
  6. Christine Meyer, Kristen Haberthur, Mark Asquith, Ilhem Messaoudi
    Pages 65-80
  7. Michael D. Leipold, Evan W. Newell, Holden T. Maecker
    Pages 81-95
  8. Feng Qian, Ruth R. Montgomery
    Pages 97-106
  9. Yair Botbol, Fernando Macian
    Pages 143-153
  10. Pradyot Dash, George C. Wang, Paul G. Thomas
    Pages 181-197
  11. Yu-Chang Wu, David Kipling, Deborah Dunn-Walters
    Pages 199-218
  12. Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J. L. Jackson, Scott D. Boyd
    Pages 219-233
  13. Alexandre de Lencastre, Frank Slack
    Pages 235-248
  14. Colin Delaney, Sanjay K. Garg, Raymond Yung
    Pages 249-264
  15. Albert C. Shaw
    Pages E1-E1
  16. Back Matter
    Pages 265-270

About this book


In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire.  In addition,  Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.


adaptive immune responses autophagy chronic disease epithelial malignancies infectious diseases innate immune responses neurodegeneration protean effects protein oxidation telomere dysfunction

Editors and affiliations

  • Albert C. Shaw
    • 1
  1. 1.Section of Infectious Diseases, Department of Internal MedicineYale School of MedicineNew HavenUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2962-7
  • Online ISBN 978-1-4939-2963-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site