Cyclin-Dependent Kinase (CDK) Inhibitors

Methods and Protocols

  • Mar Orzáez
  • Mónica Sancho Medina
  • Enrique Pérez-Payá

Part of the Methods in Molecular Biology book series (MIMB, volume 1336)

Table of contents

  1. Front Matter
    Pages i-x
  2. Reena Pinhero, Krassimir Yankulov
    Pages 13-28
  3. Asterios I. Grigoroudis, George Kontopidis
    Pages 29-45
  4. Sunil Kumar Tripathi, Poonam Singh, Sanjeev Kumar Singh
    Pages 47-58
  5. Karthiga Arumugasamy, Sunil Kumar Tripathi, Poonam Singh, Sanjeev Kumar Singh
    Pages 59-66
  6. Elena Pazos, José L. Mascareñas, M. Eugenio Vázquez
    Pages 67-83
  7. Beatriz Pérez-Benavente, Rosa Farràs
    Pages 85-93
  8. Ilaria Dutto, Micol Tillhon, Ennio Prosperi
    Pages 123-139
  9. Daniel Alvira, Laura Mondragón
    Pages 141-154
  10. Sanjiv Risal, Deepak Adhikari, Kui Liu
    Pages 155-166
  11. Hiroshi Hirai, Yoko Nakatsuru
    Pages 167-178
  12. Laura J. Hoodless, Calum T. Robb, Jennifer M. Felton, Carl S. Tucker, Adriano G. Rossi
    Pages 179-209
  13. Martina Palomino-Schätzlein, Antonio Pineda-Lucena
    Pages 211-223
  14. Back Matter
    Pages 225-227

About this book


This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.


CDK Inhibitor Proteins CDK/Cyclin Pairs Cell Culture Cyclin-Dependent Kinase Eukaryotic Cells

Editors and affiliations

  • Mar Orzáez
    • 1
  • Mónica Sancho Medina
    • 2
  • Enrique Pérez-Payá
    • 3
  1. 1.Medicinal ChemistryCentro de Investigación Príncipe FelipeValenciaSpain
  2. 2.Medicinal ChemistryCentro de Investigación Príncipe FelipeValenciaSpain
  3. 3.IBV-CSIC and Centro de Investigación Príncipe FelipeValenciaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2925-2
  • Online ISBN 978-1-4939-2926-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site