VEGF Signaling

Methods and Protocols

  • Lorna Fiedler

Part of the Methods in Molecular Biology book series (MIMB, volume 1332)

Table of contents

  1. Front Matter
    Pages i-x
  2. VEGF and VEGF Receptors

  3. Modulation and Analysis of VEGF Dependent Signaling

    1. Front Matter
      Pages 89-89
    2. Antony M. Latham, Jayakanth Kankanala, Colin W. G. Fishwick, Sreenivasan Ponnambalam
      Pages 121-131
    3. Gopika N. Nithianandarajah-Jones, Michael J. Cross
      Pages 133-142
    4. Ian Evans
      Pages 143-150
  4. In Vivo Models to Study VEGF Signaling

    1. Front Matter
      Pages 159-159
    2. Vivienne C. Ho, Guo-Hua Fong
      Pages 161-176
    3. Vedanta Mehta, David J. Carr, Anna Swanson, Anna L. David
      Pages 189-196
    4. David J. Carr, Vedanta Mehta, Jacqueline M. Wallace, Anna L. David
      Pages 197-204
    5. Linlin Yin, Li-En Jao, Wenbiao Chen
      Pages 205-217
  5. Back Matter
    Pages 219-225

About this book


This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of this book is that each chapter not only contains methodological detail rarely seen in other literature, but also contains a section on key notes and troubleshooting advice. Part I focuses on quantification of specific VEGF and VEGF receptor isoforms, beginning with a summary on VEGF splice variants, their function, and their regulation. Part II relates to the study of VEGF dependent signaling in vitro, starting with a comprehensive overview of VEGF-mediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, VEFG Signaling: Methods and Protocols is a useful tool for researching and understanding the basic biology of VEGF signaling, and translating it into the clinic.


Cardiovascular Development Lymphatic Development VEGF Gene Therapy VEGF Receptor Isoforms VEGF Receptor Trafficking VEGF Signaling Pathways Vascular Endothelial Growth Factor Vascular Permeability Factor

Editors and affiliations

  • Lorna Fiedler
    • 1
  1. 1.Caversham, ReadingUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2916-0
  • Online ISBN 978-1-4939-2917-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site