The BAM Complex

Methods and Protocols

  • Susan Buchanan
  • Nicholas Noinaj

Part of the Methods in Molecular Biology book series (MIMB, volume 1329)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Denisse L. Leyton, Matthew J. Belousoff, Trevor Lithgow
    Pages 1-16
  3. Thomas Ulrich, Philipp Oberhettinger, Ingo B. Autenrieth, Doron Rapaport
    Pages 17-31
  4. Martine P. Bos, Ria Tommassen-van Boxtel, Jan Tommassen
    Pages 33-49
  5. Nicholas Noinaj, Adam J. Kuszak, Susan K. Buchanan
    Pages 51-56
  6. Ashlee M. Plummer, Dennis Gessmann, Karen G. Fleming
    Pages 57-65
  7. Enguo Fan, Derrick Norell, Matthias Müller
    Pages 111-125
  8. Zachary C. Ruhe, Christopher S. Hayes, David A. Low
    Pages 127-136
  9. Adam J. Kuszak, Nicholas Noinaj, Susan K. Buchanan
    Pages 137-147
  10. Pamela Arden Doerner, Marcelo Carlos Sousa
    Pages 149-155
  11. Suraaj Aulakh, Kelly H. Kim, Mark Paetzel
    Pages 179-188
  12. Giselle Roman-Hernandez, Harris D. Bernstein
    Pages 203-213
  13. Chaille T. Webb, Trevor Lithgow
    Pages 215-225

About this book

Introduction

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

Keywords

BAM complex Gram-negative bacteria crystallization and structure determination functional complex individual Bam components mitochondria

Editors and affiliations

  • Susan Buchanan
    • 1
  • Nicholas Noinaj
    • 2
  1. 1.National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of HealthLaboratory of Molecular Biology, BethesdaUSA
  2. 2.Department of Biological Sciences,Markey Center for Structural Biology, Purdue UniversityWest LafayetteUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2871-2
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2870-5
  • Online ISBN 978-1-4939-2871-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book