Malaria Vaccines

Methods and Protocols

  • Ashley Vaughan

Part of the Methods in Molecular Biology book series (MIMB, volume 1325)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Pre-erythrocytic Stages

    1. Front Matter
      Pages 1-1
    2. Isaac Mohar, Katherine J. Brempelis, Sara A. Murray, Mohammad R. Ebrahimkhani, I. Nicholas Crispe
      Pages 3-17
    3. Jenna J. Guthmiller, Ryan A. Zander, Noah S. Butler
      Pages 19-37
    4. Alyse N. Douglass, Peter G. Metzger, Stefan H. I. Kappe, Alexis Kaushansky
      Pages 49-58
    5. Lander Foquet, Philip Meuleman, Cornelus C. Hermsen, Robert Sauerwein, Geert Leroux-Roels
      Pages 59-68
    6. Brandon K. Sack, Jessica L. Miller, Ashley M. Vaughan, Stefan H. I. Kappe
      Pages 69-80
  3. Mosquito Stages

    1. Front Matter
      Pages 91-91
    2. Jetsumon Sattabongkot, Chalermpon Kumpitak, Kirakorn Kiattibutr
      Pages 93-99
    3. Will J. R. Stone, Teun Bousema
      Pages 101-112
  4. Erythrocytic Stages

    1. Front Matter
      Pages 113-113
    2. Elizabeth H. Duncan, Elke S. Bergmann-Leitner
      Pages 153-165
    3. Tracy Saveria, Patrick E. Duffy, Michal Fried
      Pages 231-239
  5. Parasite Manipulation

    1. Front Matter
      Pages 255-255
    2. Ahmed M. Salman, Catherin Marin Mogollon, Jing-wen Lin, Fiona J. A. van Pul, Chris J. Janse, Shahid M. Khan
      Pages 257-286
  6. Vaccination

    1. Front Matter
      Pages 287-287
    2. Elke S. Bergmann-Leitner, Wolfgang W. Leitner
      Pages 289-302
  7. Back Matter
    Pages 303-305

About this book


This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists. The chapters within this book talk about interventions concerning all aspects of life cycle progression--measuring antibody responses to blood stage parasite survival, the T Cell responses engendered by attenuated sporozoite vaccinations, and the unique effect on transmission of antibodies that target the mosquito stage of the life cycle. Additionally, methods concerning the ability to generate targeted gene deletions and replacements in the genome of Plasmodium parasites convey how Plasmodium parasite phenotypes can be created to precise specifications. More recently, the potential power of humanized mouse models of disease progression have been demonstrated and are also discussed in the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Malaria Vaccines: Methods and Protocols is a useful and unique resource for any aspiring malaria vaccinologists because of the volume's exquisitely detailed methodologies that are not typically found in published literature.  


eukaryotic infectious disease malaria plasmodium berghei plasmodium falciparum sporozoite vaccine

Editors and affiliations

  • Ashley Vaughan
    • 1
  1. 1.Center for Infectious Disease ResearchSeattleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2814-9
  • Online ISBN 978-1-4939-2815-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site