T-Cell Development

Methods and Protocols

  • Rémy Bosselut
  • Melanie S. Vacchio

Part of the Methods in Molecular Biology book series (MIMB, volume 1323)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Background Information

    1. Front Matter
      Pages 1-1
    2. Melanie S. Vacchio, Thomas Ciucci, Rémy Bosselut
      Pages 3-21
  3. Core Approaches and Strategies

    1. Front Matter
      Pages 33-33
    2. Thomas Ciucci, Melanie S. Vacchio, Rémy Bosselut
      Pages 35-45
    3. Jan Y. M. Lee, Paul E. Love
      Pages 47-64
    4. Andrea C. Carpenter, Jong Kyong Kim, Rémy Bosselut
      Pages 87-97
    5. Amie Simmons, José Alberola-Ila
      Pages 99-108
    6. Francis A. Flomerfelt, Ronald E. Gress
      Pages 109-115
    7. Elizabeth A. Wohlfert, Andrea C. Carpenter, Yasmine Belkaid, Rémy Bosselut
      Pages 117-128
  4. Specific Techniques

    1. Front Matter
      Pages 129-129
    2. Jenny O. Ross, Heather J. Melichar, Joanna Halkias, Ellen A. Robey
      Pages 131-140
    3. Cody A. Cunningham, Emma Teixeiro, Mark A. Daniels
      Pages 141-149
    4. Zimu Deng, Haifeng Liu, Jinxiu Rui, Xiaolong Liu
      Pages 151-158
    5. Mahmood Mohtashami, Payam Zarin, Juan Carlos Zúñiga-Pflücker
      Pages 159-167
    6. Cody A. Cunningham, Emma Teixeiro, Mark A. Daniels
      Pages 169-178
    7. Levi J. Rupp, Liang Chen, Michael S. Krangel, Craig H. Bassing
      Pages 179-202
    8. Sugata Manna, Avinash Bhandoola
      Pages 203-209
    9. Francis A. Flomerfelt, Ronald E. Gress
      Pages 211-220
    10. Inge Van de Walle, Karin Davids, Tom Taghon
      Pages 221-237
    11. Anne-Catherine Dolens, Inge Van de Walle, Tom Taghon
      Pages 239-251
    12. Sarah Bonte, Sylvia Snauwaert, Stijn Vanhee, Anne-Catherine Dolens, Tom Taghon, Bart Vandekerckhove et al.
      Pages 253-272
    13. Yong Zhang, David L. Wiest
      Pages 273-292
  5. Back Matter
    Pages 293-297

About this book


This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.


bone marrow chimera techniques culture of thymic fetal thymic organ culture generating thymic epithelial in vitro techniques live imaging techniques organ culture thymic slices thymus

Editors and affiliations

  • Rémy Bosselut
    • 1
  • Melanie S. Vacchio
    • 2
  1. 1.Laboratory of Immune Cell BiologyNational Institutes of Health National Cancer InstituteBethesdaUSA
  2. 2.Laboratory of Immune Cell BiologyNational Institutes of Health National Cancer InstituteBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2808-8
  • Online ISBN 978-1-4939-2809-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site