Methods and Protocols

  • Alexandra Castilho

Part of the Methods in Molecular Biology book series (MIMB, volume 1321)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Glyco-Engineering in Bacteria

    1. Front Matter
      Pages 1-1
    2. Stephen R. P. Jaffé, Benjamin Strutton, Jagroop Pandhal, Phillip C. Wright
      Pages 17-35
    3. Nicolas F. Vozza, Mario F. Feldman
      Pages 57-70
  3. Glyco-Engineering in Yeast

  4. Glyco-Engineering in Insects

    1. Front Matter
      Pages 129-129
    2. Christoph Geisler, Hideaki Mabashi-Asazuma, Donald L. Jarvis
      Pages 131-152
    3. Karthik Viswanathan, Someet Narang, Michael J. Betenbaugh
      Pages 171-178
  5. Glyco-Engineering in Plants

  6. Glyco-Engineering in Mammalians

    1. Front Matter
      Pages 285-285
    2. Qiong Wang, Mark Stuczynski, Yuan Gao, Michael J. Betenbaugh
      Pages 287-305
    3. Veronica T. Chang, Robert A. Spooner, Max Crispin, Simon J. Davis
      Pages 307-322
    4. Friedrich Piller, Aline Mongis, Véronique Piller
      Pages 335-359
    5. Katrin Braasch, Carina Villacrés, Michael Butler
      Pages 361-372
  7. In Vitro Glyco-Engineering of Recombinant Proteins

    1. Front Matter
      Pages 373-373
    2. John P. Giddens, Lai-Xi Wang
      Pages 375-387
    3. Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, Alexander G. Gabibov
      Pages 389-404
  8. Glycan Analysis

    1. Front Matter
      Pages 405-405
    2. Christina E. Galuska, Kai Maass, Sebastian P. Galuska
      Pages 417-426
    3. Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, Friedrich Altmann
      Pages 427-435
  9. Back Matter
    Pages 437-439

About this book


Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants, and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.


Carbohydrate composition Glycan analysis Glyco-designed proteins Glycoproteins Glycosylation pathway N-linked Production host manipulation

Editors and affiliations

  • Alexandra Castilho
    • 1
  1. 1.University of Natural Resources and Life SciencesViennaAustria

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2759-3
  • Online ISBN 978-1-4939-2760-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site