Methods and Protocols

  • Robert Hnasko

Part of the Methods in Molecular Biology book series (MIMB, volume 1318)

Table of contents

  1. Front Matter
    Pages i-x
  2. Robert M. Hnasko, Larry H. Stanker
    Pages 15-28
  3. Robert M. Hnasko, Jeffery A. McGarvey
    Pages 29-41
  4. Alice V. Lin
    Pages 51-59
  5. Alice V. Lin
    Pages 61-67
  6. Thomas S. Hnasko, Robert M. Hnasko
    Pages 87-96
  7. Andrew Lin, Alexandra Salvador, J. Mark Carter
    Pages 107-118
  8. Laurie M. Clotilde, Heng Yu, M. Luis Carbonell
    Pages 119-126
  9. Kathryn H. Ching
    Pages 127-137
  10. Xiaohua He, Stephanie A. Patfield
    Pages 139-148
  11. Sonchita Bagchi, Robert Fredriksson, Åsa Wallén-Mackenzie
    Pages 149-159
  12. Lauren Faget, Thomas S. Hnasko
    Pages 161-172
  13. Khoren Sahagian, Mikki Larner
    Pages 197-214
  14. Back Matter
    Pages 215-216

About this book


This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample.  The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production.  An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies.  Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems.  The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.


Enzyme-Linked Immunosorbent Assay antibody hybridoma technology immunoassay strategies monoclonal antibodies

Editors and affiliations

  • Robert Hnasko
    • 1
  1. 1.Agricultural Research ServiceUSDA-ARSAlbanyUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2741-8
  • Online ISBN 978-1-4939-2742-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site