Detection of Blotted Proteins

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 1314)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Vishal Kothari, Suresh T. Mathews
    Pages 27-32
  3. Anil Dsouza, R. Hal Scofield
    Pages 33-40
  4. F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, Joan-Ramon Daban
    Pages 41-50
  5. Neil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman, Keith R. Olson
    Pages 51-61
  6. Claudia Franke, Daniel Gräfe, Holger Bartsch, Michael P. Bachmann
    Pages 63-71
  7. Holger Bartsch, Claudia Franke, Michael P. Bachmann
    Pages 73-78
  8. Surbhi Desai, Boguslawa R. Dworecki, Marie C. Nlend
    Pages 101-114
  9. Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, Michael J. Dunn
    Pages 139-149
  10. Biji T. Kurien, Michael P. Bachmann
    Pages 165-171

About this book

Introduction

This detailed volume provides methods and techniques for detection after blotting.  Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.

Keywords

detecting blotted molecular species detecting the blotted proteins protein blotting protein transfer western blotting techniques

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Arthritis and Clinical Immunology ProgramOklahoma Medical Research FoundationOklahoma CityUSA
  2. 2.Department of MedicineOklahoma Medical Research FoundationOklahoma CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2718-0
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2717-3
  • Online ISBN 978-1-4939-2718-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book