Methods and Protocols

  • Ulrich Lehmann
  • Jörg Tost

Part of the Methods in Molecular Biology book series (MIMB, volume 1315)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Pål Nyrén
      Pages 3-15
    3. Martin Kreutz, Gerald Schock, Julia Kaiser, Norbert Hochstein, Ralf Peist
      Pages 17-27
    4. Guoli Chen, Matthew T. Olson, James R. Eshleman
      Pages 29-38
  3. Analysis of Genetic Variation

    1. Front Matter
      Pages 47-47
    2. Hui Ye, Haiping Wu, Yunlong Liu, Bingjie Zou, Tomoharu Kajiyama, Hideki Kambara et al.
      Pages 123-132
    3. Irene Vanni, Elisabetta Ugolotti, Patrizia Larghero, Roberto Biassoni
      Pages 133-151
  4. Analysis of DNA Methylation Patterns

    1. Front Matter
      Pages 173-173
    2. Jessica Roessler, Ulrich Lehmann
      Pages 175-188
    3. Barbara Banelli, Massimo Romani
      Pages 189-200
    4. Ali M. Tabish, Andrea A. Baccarelli, Lode Godderis, Timothy M. Barrow, Peter Hoet, Hyang-Min Byun
      Pages 201-207
    5. Karin Luttropp, Louise K. Sjöholm, Tomas J. Ekström
      Pages 209-219
    6. Silvia Tabano, Eleonora Bonaparte, Monica Miozzo
      Pages 241-258
    7. John Huntriss, Kathryn Woodfine, Joanna E. Huddleston, Adele Murrell, Helen M. Picton
      Pages 259-270
    8. Lasse Sommer Kristensen, Jens Vilstrup Johansen, Kirsten Grønbæk
      Pages 271-289
    9. Céline Moison, Fanny Assemat, Antoine Daunay, Paola B. Arimondo, Jörg Tost
      Pages 315-333
  5. Bacterial Typing and Identification

    1. Front Matter
      Pages 335-335
    2. Piotr Wojciech Dabrowski, Kati Bourquain, Andreas Nitsche
      Pages 337-347
    3. Anna Engström, Pontus Juréen
      Pages 349-362
    4. Kingsley Kwaku Amoako
      Pages 363-375
  6. Forensics

    1. Front Matter
      Pages 377-377
    2. Magdalena M. Bus´, Hanna Edlund, Marie Allen
      Pages 379-396
    3. Joana Antunes, Kuppareddi Balamurugan, George Duncan, Bruce McCord
      Pages 397-409
  7. Back Matter
    Pages 411-414

About this book


The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators, and research scientists studying different aspects of genetics and cellular and molecular biology.


DNA methylation HLA alleles Pyrograms allele-specific DNA methylation patterns alleles chromosomal alterations genetic variation genomics medical diagnostics viruses and bacteria

Editors and affiliations

  • Ulrich Lehmann
    • 1
  • Jörg Tost
    • 2
  1. 1.Medizinische Hochschule Hannover, Institute of PathologyHannoverGermany
  2. 2.Laboratory for Epigenetics and EnvironmentCentre National de Génotypage, CEA- Institut de GénomiqueEvryFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2714-2
  • Online ISBN 978-1-4939-2715-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site