© 2015


Methods and Protocols

  • Magnus Lundgren
  • Emmanuelle Charpentier
  • Peter C. Fineran

Part of the Methods in Molecular Biology book series (MIMB, volume 1311)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Nadja Heidrich, Gaurav Dugar, Jörg Vogel, Cynthia M. Sharma
    Pages 1-21
  3. André Plagens, Lennart Randau
    Pages 23-33
  4. Erin L. Garside, Andrew M. MacMillan
    Pages 35-46
  5. Kira S. Makarova, Eugene V. Koonin
    Pages 47-75
  6. Ambarish Biswas, Peter C. Fineran, Chris M. Brown
    Pages 77-89
  7. Christophe Sola, Edgar Abadia, Simon Le Hello, François-Xavier Weill
    Pages 91-109
  8. Igor Mokrousov, Nalin Rastogi
    Pages 111-131
  9. Sakharam P. Waghmare, Alison O. Nwokeoji, Mark J. Dickman
    Pages 133-145
  10. Alexandra Strotksaya, Ekaterina Semenova, Ekaterina Savitskaya, Konstantin Severinov
    Pages 147-159
  11. Tim Künne, Edze R. Westra, Stan J. J. Brouns
    Pages 171-184
  12. Marie-Ève Dupuis, Rodolphe Barrangou, Sylvain Moineau
    Pages 195-222
  13. Ido Yosef, Moran G. Goren, Rotem Edgar, Udi Qimron
    Pages 233-250
  14. Natalia Beloglazova, Sofia Lemak, Robert Flick, Alexander F. Yakunin
    Pages 251-264
  15. Tomas Sinkunas, Giedrius Gasiunas, Virginijus Siksnys
    Pages 277-291

About this book


This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.


CRISPR-Cas defense systems CRISPR-Cas9 technology CRISPR-based genetic tools DNA/protein RNA/protein archaeal species bacterial species genetic elements horizontal gene transfer protein/protein interactions

Editors and affiliations

  • Magnus Lundgren
    • 1
  • Emmanuelle Charpentier
    • 2
  • Peter C. Fineran
    • 3
  1. 1.Department of Cell and Molecular BiologyUppsala UniversityUppsalaSweden
  2. 2.Department of Molecular Biology MIMSUmeå UniversityUmeåSweden
  3. 3.Department of Microbiology and ImmunologyUniversity of OtagoDunedinNew Zealand

Bibliographic information

  • Book Title CRISPR
  • Book Subtitle Methods and Protocols
  • Editors Magnus Lundgren
    Emmanuelle Charpentier
    Peter C. Fineran
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-2686-2
  • Softcover ISBN 978-1-4939-5428-5
  • eBook ISBN 978-1-4939-2687-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 366
  • Number of Illustrations 26 b/w illustrations, 37 illustrations in colour
  • Topics Gene Expression
  • Buy this book on publisher's site