Methods and Protocols

  • Gary R. Skuse
  • Maureen C. Ferran

Part of the Methods in Molecular Biology book series (MIMB, volume 1299)

Table of contents

  1. Front Matter
    Pages i-x
  2. Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, Rama S. Verma
    Pages 17-25
  3. Nancy K. Drew, Anna Grosberg
    Pages 75-91
  4. Carley Glass, Reetu Singla, Anshu Arora, Dinender K. Singla
    Pages 145-152
  5. Adam C. Vandergriff, M. Taylor Hensley, Ke Cheng
    Pages 153-160
  6. Winston Shim, Haiyang Yu, K. P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, Philip Wong
    Pages 167-175
  7. Sarah E. Lang, Margaret V. Westfall
    Pages 177-190
  8. Back Matter
    Pages 213-216

About this book


This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics, and function of cardiomyocytes.  The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes, and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.


Cardiac myocyte gene transfer Cardiac tissue repair Cardiomyopathies Cardiovascular disease Gene expression analysis Heart disease Whole organ generation

Editors and affiliations

  • Gary R. Skuse
    • 1
  • Maureen C. Ferran
    • 2
  1. 1.Rochester Institute of TechnologyRochesterUSA
  2. 2.Rochester Institute of TechnologyRochesterUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2571-1
  • Online ISBN 978-1-4939-2572-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site