Chromatin Protocols

  • Srikumar P. Chellappan

Part of the Methods in Molecular Biology book series (MIMB, volume 1288)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Olga I. Kulaeva, Vasily M. Studitsky
    Pages 15-26
  3. Yuri L. Lyubchenko, Luda S. Shlyakhtenko
    Pages 27-42
  4. Lirong Peng, Zhigang Yuan, Edward Seto
    Pages 95-108
  5. Kenji Kokura, Lidong Sun, Jia Fang
    Pages 109-122
  6. Carolina E. Pardo, Nancy H. Nabilsi, Russell P. Darst, Michael P. Kladde
    Pages 123-141
  7. Heui-Yun Joo, Qian Dai, Amanda E. Jones, Ling Zhai, Hengbin Wang
    Pages 213-230
  8. Sujata Kumari, Chandrima Das, Sweta Sikder, Manoj Kumar, Mahesh Bachu, Udaykumar Ranga et al.
    Pages 245-272
  9. Hélène Gaillard, Ralf Erik Wellinger, Andrés Aguilera
    Pages 273-288
  10. Durga Udayakumar, Nobuo Horikoshi, Lopa Mishra, Clayton Hunt, Tej K. Pandita
    Pages 317-336
  11. Salomé Adam, Juliette Dabin, Siau-Kun Bai, Sophie E. Polo
    Pages 337-347
  12. Wasia Rizwani, Srikumar P. Chellappan
    Pages 349-362
  13. Sergey Shaposhnikov, Naouale El Yamani, Andrew R. Collins
    Pages 363-373
  14. Parijat Senapati, Deepthi Sudarshan, Shrikanth S. Gadad, Jayasha Shandilya, Venkatesh Swaminathan, Tapas K. Kundu
    Pages 375-394
  15. Kseniya S. Kudryashova, Oleg V. Chertkov, Dmitry V. Nikitin, Nikolai A. Pestov, Olga I. Kulaeva, Anastasija V. Efremenko et al.
    Pages 395-412
  16. Edgar González-Buendía, Ricardo Saldaña-Meyer, Karin Meier, Félix Recillas-Targa
    Pages 413-428
  17. Back Matter
    Pages 489-492

About this book


Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation, and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin.  Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.


Chromatin imaging Chromatin structure and function DNA methylation Histone modifications Non-histone chromatin proteins Nucleosomes Transcriptional regulation

Editors and affiliations

  • Srikumar P. Chellappan
    • 1
  1. 1.H. Lee Moffitt Cancer Center & Research InstituteTampaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2473-8
  • Online ISBN 978-1-4939-2474-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site