Plant Gene Silencing

Methods and Protocols

  • Kirankumar S. Mysore
  • Muthappa Senthil-Kumar

Part of the Methods in Molecular Biology book series (MIMB, volume 1287)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Review Chapters

    1. Front Matter
      Pages 1-1
    2. Prachi Pandey, Muthappa Senthil-Kumar, Kirankumar S. Mysore
      Pages 3-23
    3. Firoz Ahmed, Xinbin Dai, Patrick Xuechun Zhao
      Pages 43-60
    4. Dongdong Niu, Zhaoyun Wang, Shune Wang, Lulu Qiao, Hongwei Zhao
      Pages 61-79
    5. Abdulrazak B. Ibrahim, Francisco J. L. Aragão
      Pages 81-92
  3. Methods Chapter

    1. Front Matter
      Pages 93-93
    2. Vibha Srivastava, David W. Ow
      Pages 95-103
    3. Yun Jin, Hui-Shan Guo
      Pages 105-117
    4. Masamitsu Wada, Hidenori Tsuboi
      Pages 119-127
    5. Jinggui Fang, Xin Sun
      Pages 139-145
    6. Weiwei Chen, Qi Zhang, Junhua Kong, Feng Hu, Bin Li, Chaoqun Wu et al.
      Pages 147-157
    7. John R. Tuttle, Candace H. Haigler, Dominique (Niki) Robertson
      Pages 219-234
    8. Gavin M. George, Michael E. Ruckle, James R. Lloyd
      Pages 243-253
    9. Maoying Li, Fangjun Li, Ping He
      Pages 267-279
    10. M. E. Chrissie Rey, Johan Harmse, Sarah H. Taylor, Patrick Arbuthnot, Marc S. Weinberg
      Pages 295-304
  4. Back Matter
    Pages 305-307

About this book


This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.


DNA interference RNA interference RNA-induced silencing complex double stranded RNA gene regulation hairpin RNA micro RNA post transcriptional gene silencing transcriptional gene silencing virus-induced gene silencing

Editors and affiliations

  • Kirankumar S. Mysore
    • 1
  • Muthappa Senthil-Kumar
    • 2
  1. 1.Plant Biology Division, The Samuel Roberts Noble FoundationArdmoreUSA
  2. 2.National Institute of Plant Genome ResearchNew DelhiIndia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2452-3
  • Online ISBN 978-1-4939-2453-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site