Affinity Chromatography

Methods and Protocols

  • Senta Reichelt

Part of the Methods in Molecular Biology book series (MIMB, volume 1286)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. David S. Hage, Ryan Matsuda
    Pages 1-19
  3. General Modes and Ligands of Affinity Chromatography

    1. Front Matter
      Pages 21-21
    2. Vânia C. Graça, Fani Sousa, Paulo F. Santos, Paulo S. Almeida
      Pages 23-33
    3. Cunxi Wang, Thomas C. Lee, Kathleen S. Crowley, Erin Bell
      Pages 35-42
    4. Handan Yavuz, Nilay Bereli, Fatma Yılmaz, Canan Armutcu, Adil Denizli
      Pages 43-46
    5. Yan Gao, Wenjie Li, Ye Zhang, Dimitri Pappas
      Pages 55-65
    6. Guohong Zhu, Johanna-Gabriela Walter
      Pages 67-82
    7. Simone C. Wiesler, Robert O. J. Weinzierl
      Pages 97-106
  4. Matrices in Affinity Chromatography

  5. Macroporous Polymers as Matrix in Affinity Chromatography

    1. Front Matter
      Pages 171-171
    2. Senta Reichelt
      Pages 173-181
    3. Solmaz Hajizadeh, Bo Mattiasson
      Pages 183-200
    4. Naveen Kumar Singh, Roy N. DSouza, Noor Shad Bibi, Marcelo Fernández-Lahore
      Pages 201-212
    5. Muge Andac, Igor Yu Galaev, Handan Yavuz, Adil Denizli
      Pages 233-237
  6. Spectroscopy, Binding Studies and Molecular Modelling

  7. Back Matter
    Pages 337-341

About this book


The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols,  molecular modelling,  and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.



Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is  designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.


binding constants cryogelation process dye affinity chromatography immobilized metal-chelated affinity chromatography kinetic rate macroporous monoliths mass transfer polymeric network quantitative isolation of biomolecules

Editors and affiliations

  • Senta Reichelt
    • 1
  1. 1.Leibniz-Institut für OberflächenmodifizierungLeipzigGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2446-2
  • Online ISBN 978-1-4939-2447-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site