NF-kappa B

Methods and Protocols

  • Michael J. May

Part of the Methods in Molecular Biology book series (MIMB, volume 1280)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Standard Approaches to Detect NF-κB Pathway Activation

    1. Front Matter
      Pages 1-1
    2. Sitharam Ramaswami, Matthew S. Hayden
      Pages 3-13
    3. Petro Starokadomskyy, Ezra Burstein
      Pages 15-24
  3. Detection and Analysis of NF-κB Signaling

  4. Methods to Study the Control of NF-κB Signaling

    1. Front Matter
      Pages 217-217
    2. Daniel Nagel, Daniel Krappmann
      Pages 239-246
    3. Anna D. Reichardt, Jose Pindado, Shivam A. Zaver, Genhong Cheng
      Pages 247-268
    4. Eugene Varfolomeev, Tatiana Goncharov, Domagoj Vucic
      Pages 269-282
    5. Yoshiteru Sasaki, Hiroaki Fujita, Misa Nakai, Kazuhiro Iwai
      Pages 297-309
    6. Michelle Vincendeau, Daniel Krappmann, Kamyar Hadian
      Pages 311-320
    7. Robert A. Chong, Kenneth Wu, Jordan Kovacev, Zhen-Qiang Pan
      Pages 339-354
    8. Patricia E. Collins, Amy Colleran, Ruaidhrí J. Carmody
      Pages 355-370
    9. Haiying Li, Petro Starokadomskyy, Ezra Burstein
      Pages 371-381
    10. JinJing Chen, Lin-Feng Chen
      Pages 395-409
  5. Analyzing and Targeting NF-κB Activity in Disease

    1. Front Matter
      Pages 411-411
    2. Daniela S. Bassères, Albert S. Baldwin
      Pages 447-458
    3. Clint T. Allen, Carter Van Waes
      Pages 459-468
    4. Anita Gaurnier-Hausser, Nicola J. Mason
      Pages 469-504
    5. Gaurav Swarnkar, Yousef Abu-Amer
      Pages 527-542
    6. Jing Zhao, Xuesen Li, Sara McGowan, Laura J. Niedernhofer, Paul D. Robbins
      Pages 543-557
    7. Bettina Sehnert, Harald Burkhardt, Stefan Dübel, Reinhard E. Voll
      Pages 559-578
    8. Bettina Sehnert, Harald Burkhardt, Michael J. May, Jochen Zwerina, Reinhard E. Voll
      Pages 579-591
    9. Helena Shaked, Monica Guma, Michael Karin
      Pages 593-606
  6. Bioinformatics and Modeling Approaches to Study NF-κB

    1. Front Matter
      Pages 607-607
    2. Trevor Siggers, Thomas D. Gilmore, Brian Barron, Ashley Penvose
      Pages 609-630
    3. Simon Mitchell, Rachel Tsui, Alexander Hoffmann
      Pages 647-661
  7. Back Matter
    Pages 663-668

About this book


Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.


DELFIA Electrophoretic Mobility Shift Epidermal Growth Factor (EGF) IKK activity NF-κB pathway activation NF-κB pathway signaling proteins T cell receptor (TCR) chromatin immunoprecipitation (ChIP) microscale thermophoresis non-canonical NF-κB pathway

Editors and affiliations

  • Michael J. May
    • 1
  1. 1.Department of Animal BiologyUniversity of Pennsylvania School of Veterinary MedicinePhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2421-9
  • Online ISBN 978-1-4939-2422-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site