Bacterial Transcriptional Control

Methods and Protocols

  • Irina Artsimovitch
  • Thomas J. Santangelo

Part of the Methods in Molecular Biology book series (MIMB, volume 1276)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Vladimir Svetlov, Irina Artsimovitch
    Pages 13-29
  3. Anssi M. Malinen, Matti Turtola, Georgiy A. Belogurov
    Pages 31-51
  4. Martin Buck, Christoph Engl, Nicolas Joly, Goran Jovanovic, Milija Jovanovic, Edward Lawton et al.
    Pages 53-79
  5. Anirban Chakraborty, Abhishek Mazumder, Miaoxin Lin, Adam Hasemeyer, Qumiao Xu, Dongye Wang et al.
    Pages 101-131
  6. Lucyna Lubkowska, Maria L. Kireeva
    Pages 153-164
  7. Irina O. Vvedenskaya, Seth R. Goldman, Bryce E. Nickels
    Pages 211-228
  8. A. Rachid Rahmouni, Christine Mosrin-Huaman
    Pages 229-240
  9. Francis Dean Appling, David Alan Schneider
    Pages 281-289
  10. Katherine Smollett, Fabian Blombach, Finn Werner
    Pages 291-303
  11. Katherine Smollett, Fabian Blombach, Finn Werner
    Pages 305-314
  12. Fu-Kai Hsieh, Olga I. Kulaeva, Vasily M. Studitsky
    Pages 315-326
  13. Richard P. Bonocora, Joseph T. Wade
    Pages 327-340
  14. Back Matter
    Pages 341-342

About this book


This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.

Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.


Bacterial RNA polymerases archaeal RNA polymerases enzyme eukaryotic RNA polymerases intermolecular movements mRNA synthesis phenotypes transcription cycle

Editors and affiliations

  • Irina Artsimovitch
    • 1
  • Thomas J. Santangelo
    • 2
  1. 1.Department of MicrobiologyThe Ohio State UniversityColumbusUSA
  2. 2.Department of Biochemistry and Molecular BiologyColorado State UniversityFort CollinsUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2391-5
  • Online ISBN 978-1-4939-2392-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site