Methods and Protocols

  • Beata Jastrzebska

Part of the Methods in Molecular Biology book series (MIMB, volume 1271)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Historical Overview

    1. Front Matter
      Pages 1-1
    2. Lukas Hofmann, Krzysztof Palczewski
      Pages 3-18
  3. Rhodopsin Expression, Regeneration, and Purification for Structural Studies

    1. Front Matter
      Pages 19-19
    2. Elise Blankenship, David T. Lodowski
      Pages 21-38
    3. Daniel Mattle, Ankita Singhal, Georg Schmid, Roger Dawson, Jörg Standfuss
      Pages 39-54
    4. Grazyna Palczewska, David Salom
      Pages 55-64
  4. Structure–Function Characterization of Rhodopsin

    1. Front Matter
      Pages 65-65
    2. Qiuyan Chen, Sergey A. Vishnivetskiy, Tiandi Zhuang, Min-Kyu Cho, Tarjani M. Thaker, Charles R. Sanders et al.
      Pages 77-95
    3. Andrey V. Struts, Udeep Chawla, Suchithranga M. D. C. Perera, Michael F. Brown
      Pages 133-158
    4. Naoki Kimata, Andreyah Pope, Dawood Rashid, Philip J. Reeves, Steven O. Smith
      Pages 159-171
    5. Paul S.-H. Park, Daniel J. Müller
      Pages 173-185
  5. Rhodopsin Supramolecular Organization and Its Complexes with Partner Proteins

  6. Rhodopsin and Photoreceptors

    1. Front Matter
      Pages 265-265
    2. Theodore G. Wensel, Jared C. Gilliam
      Pages 267-292
    3. Kerrie H. Lodowski, Yoshikazu Imanishi
      Pages 293-307
  7. Treatment Strategies of Retinal Degenerative Disease

    1. Front Matter
      Pages 325-325
    2. Leopold Adler IV, Nicholas P. Boyer, Chunhe Chen, Yiannis Koutalos
      Pages 327-343
    3. Peter H. Tang, Rosalie K. Crouch
      Pages 363-368
    4. Brian P. Rossmiller, Renee C. Ryals, Alfred S. Lewin
      Pages 391-410
  8. Back Matter
    Pages 411-414

About this book


This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first chapters of the book focus on methods developed to study fundamentals of rhodopsin structure and function, starting with improved purification protocols of native and mutated rhodopsin, followed by methods used for rhodopsin reconstitution into lipid bilayers stabilizing rhodopsin function properties, and finally describing recently developed methods to study structural dynamics of rhodopsin activation and its mechanistic properties. Subsequently, chapters underline various techniques that have been developed to visualize the rhodopsin dimer and to study its functional significance.  The next few chapters highlight cutting-edge imaging techniques of photoreceptors, rhodopsin trafficking, and its diffusion within signaling membranes. Finally the book concludes with recent developments that could be potentially beneficial in patient treatments, and treatment strategies for retinal degenerative diseases. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Timely and practical, Rhodopsin: Methods and Protocols reaches out to researchers and health practitioners, and provides timely protocol useful for studying  structural and functional properties of rhodopsin.


G-Protein Coupled Receptor age-related macular degeneration photoreceptor degenerative diseases protein crystallization protein engineering purifications protocols rhodopsin rhodopsin dimer structural dynamics

Editors and affiliations

  • Beata Jastrzebska
    • 1
  1. 1.Department of Pharmacology, School of MedicineCase Western Reserve UniversityClevelandUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2329-8
  • Online ISBN 978-1-4939-2330-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site