Site-Specific Protein Labeling

Methods and Protocols

  • Arnaud Gautier
  • Marlon J. Hinner

Part of the Methods in Molecular Biology book series (MIMB, volume 1266)

Table of contents

  1. Front Matter
    Pages i-xii
  2. B. Albert Griffin, Stephen R. Adams, Roger Y. Tsien
    Pages 1-6
  3. Gražvydas Lukinavičius, Luc Reymond, Kai Johnsson
    Pages 107-118
  4. Hélène A. Benink, Marjeta Urh
    Pages 119-128
  5. Julian C. J. Matern, Anne-Lena Bachmann, Ilka V. Thiel, Gerrit Volkmann, Alexandra Wasmuth, Jens Binschik et al.
    Pages 129-143
  6. Anne-Lena Bachmann, Julian C. J. Matern, Vivien Schütz, Henning D. Mootz
    Pages 145-159
  7. Bo Zhao, Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Han Zhou, Mengnan Zhang et al.
    Pages 161-170
  8. Michael Fairhead, Mark Howarth
    Pages 171-184
  9. Peter Landgraf, Elmer R. Antileo, Erin M. Schuman, Daniela C. Dieterich
    Pages 199-215
  10. Kathrin Lang, Lloyd Davis, Jason W. Chin
    Pages 217-228
  11. Tomonori Tamura, Itaru Hamachi
    Pages 229-242
  12. Back Matter
    Pages 265-267

About this book

Introduction

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters present a comprehensive overview on the most relevant and established labeling methodologies, and help researchers to choose the most appropriate labeling method for their biological question. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols is designed to help scientists from all fields to get a better understanding of labeling techniques.

Keywords

CLIP-tag technology FlAsH-tag SNAP-tag intracellular proteins labeled proteins membrane translocation protein translation site-specific protein labeling tag-based methods unnatural amino acids

Editors and affiliations

  • Arnaud Gautier
    • 1
  • Marlon J. Hinner
    • 2
  1. 1.Department of ChemistryÉcole Normale SupérieureParisFrance
  2. 2.Sensitive Farbstoffe GbRMunichGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2272-7
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2271-0
  • Online ISBN 978-1-4939-2272-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book