Chemical Biology

Methods and Protocols

  • Jonathan E. Hempel
  • Charles H. Williams
  • Charles C. Hong

Part of the Methods in Molecular Biology book series (MIMB, volume 1263)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. In Vitro Chemical Genetic Screening

    1. Front Matter
      Pages 1-1
    2. Yasumitsu Kondoh, Kaori Honda, Hiroyuki Osada
      Pages 29-41
    3. Sean Spiering, Herman Davidovics, Paul J. Bushway, Mark Mercola, Erik Willems
      Pages 43-61
    4. Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, Ethan Lee
      Pages 63-73
  3. In Vivo Chemical Genetic Screening

    1. Front Matter
      Pages 75-75
    2. Ana Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, Charles S. Hoffman
      Pages 77-91
    3. Manal J. Natto, Anthonius A. Eze, Harry P. de Koning
      Pages 103-110
    4. Mickael Poidevin, Feiran Zhang, Peng Jin
      Pages 127-138
    5. Sunitha Rangaraju, Gregory M. Solis, Michael Petrascheck
      Pages 139-155
  4. Small Molecules in Chemical Biology

    1. Front Matter
      Pages 175-175
    2. Jingyu Zhang, Jianying Han, Ayokunmi Oyeleye, Miaomiao Liu, Xueting Liu, Lixin Zhang
      Pages 177-185
    3. Jianying Han, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu et al.
      Pages 187-196
    4. Hai-Young Kim, Daniel F. Wyss
      Pages 197-208
    5. Jonathan E. Hempel, Charles C. Hong
      Pages 209-223
    6. Sargis Dallakyan, Arthur J. Olson
      Pages 243-250
  5. Small Molecule Target Identification

    1. Front Matter
      Pages 251-251
    2. Glòria Vendrell-Navarro, Andreas Brockmeyer, Herbert Waldmann, Petra Janning, Slava Ziegler
      Pages 263-286
    3. Melody Y. Pai, Brett Lomenick, Heejun Hwang, Robert Schiestl, William McBride, Joseph A. Loo et al.
      Pages 287-298
    4. Jeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong et al.
      Pages 299-318
    5. Shinsuke Ohnuki, Hiroki Okada, Yoshikazu Ohya
      Pages 319-327
  6. Back Matter
    Pages 329-333

About this book


This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organized into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.


biological function chemical functional protein targets in vitro cellular systems in vitro-based compound screening in vivo chemical genetic screening in vivo-based compound screening phenotypes target identification

Editors and affiliations

  • Jonathan E. Hempel
    • 1
  • Charles H. Williams
    • 2
  • Charles C. Hong
    • 3
  1. 1.Department of Medicine - Cardiovascular MedicineVanderbilt Institute of Chemical Biology, Vanderbilt University School of MedicineNashvilleUSA
  2. 2.Department of Cell and Developmental BiologyVanderbilt Institute of Chemical Biology, Vanderbilt University School of MedicineNashvilleUSA
  3. 3.Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental BiologyVanderbilt Institute of Chemical Biology, Vanderbilt University School of MedicineNashvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2268-0
  • Online ISBN 978-1-4939-2269-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site