Insoluble Proteins

Methods and Protocols

  • Elena García-Fruitós

Part of the Methods in Molecular Biology book series (MIMB, volume 1258)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, Zhikun Xu, Elena García-Fruitós
    Pages 1-24
  3. Recombinant Protein Production in Escherichia coli

    1. Front Matter
      Pages 25-25
    2. Ranjana Arya, Jamal S. M. Sabir, Roop S. Bora, Kulvinder S. Saini
      Pages 45-63
    3. Zhanglin Lin, Qing Zhao, Bihong Zhou, Lei Xing, Wanghui Xu
      Pages 65-78
    4. Jason T. Boock, Dujduan Waraho-Zhmayev, Dario Mizrachi, Matthew P. DeLisa
      Pages 79-97
    5. Anna Villar-Pique, Susanna Navarro, Salvador Ventura
      Pages 99-122
  4. Strategies to Produce Insoluble Proteins in Cell-Free Expression Systems

    1. Front Matter
      Pages 123-123
    2. Lei Kai, Erika Orbán, Erik Henrich, Davide Proverbio, Volker Dötsch, Frank Bernhard
      Pages 125-143
  5. Recombinant Protein Production in Lactic Acid Bacteria (LAB)

    1. Front Matter
      Pages 145-145
    2. Sylvain Boutigny, Emeline Sautron, Annie Frelet-Barrand, Lucas Moyet, Daniel Salvi, Norbert Rolland et al.
      Pages 147-165
  6. Recombinant Protein Production in Yeast

  7. Recombinant Protein Production in Insect Cells-Baculovirus

    1. Front Matter
      Pages 179-179
    2. Judit Osz-Papai, Laura Radu, Wassim Abdulrahman, Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Catherine Birck et al.
      Pages 181-205
  8. Recombinant Protein Production in Mammalian Cells

    1. Front Matter
      Pages 207-207
    2. Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay et al.
      Pages 209-222
    3. Kamilla Swiech, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, Virgínia Picanço-Castro
      Pages 223-240
  9. Recombinant Protein Production in Other Systems

    1. Front Matter
      Pages 241-241
    2. Maria Giuliani, Ermenegilda Parrilli, Filomena Sannino, Gennaro Apuzzo, Gennaro Marino, Maria Luisa Tutino
      Pages 243-257
  10. Insoluble Protein Purification

    1. Front Matter
      Pages 259-259
    2. Mario Lebendiker, Michal Maes, Assaf Friedler
      Pages 261-281
    3. Anupam Singh, Vaibhav Upadhyay, Amulya K. Panda
      Pages 283-291
    4. Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, Elena García-Fruitós
      Pages 293-305
  11. Insoluble Protein Characterization

    1. Front Matter
      Pages 319-319
    2. Mireia Pesarrodona, Ugutz Unzueta, Esther Vázquez
      Pages 321-330
    3. Sílvia Bronsoms, Sebastián A. Trejo
      Pages 331-345
    4. Witold Tatkiewicz, Elisa Elizondo, Evelyn Moreno, Cesar Díez-Gil, Nora Ventosa, Jaume Veciana et al.
      Pages 387-401
    5. Roger G. Harrison, Miguel J. Bagajewicz
      Pages 403-408
  12. Insoluble Protein Applications

    1. Front Matter
      Pages 409-409
    2. Eva Hrabárová, Lucia Achbergerová, Jozef Nahálka
      Pages 411-422
  13. Back Matter
    Pages 423-425

About this book


With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.


Biotechnology Expression systems Insolubility Pharmaceuticals Protein production Protein purification Recombinant proteins

Editors and affiliations

  • Elena García-Fruitós
    • 1
  1. 1.Departament de Genètica i de MicrobiologiaCIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) and Universitat Autònoma de BarcelonaBellaterra (Barcelona)Spain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2204-8
  • Online ISBN 978-1-4939-2205-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site