Polyadenylation in Plants

Methods and Protocols

  • Arthur G. Hunt
  • Qingshun Quinn Li

Part of the Methods in Molecular Biology book series (MIMB, volume 1255)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Bioinformatics Studies of Plant Polyadenylation

    1. Front Matter
      Pages 1-1
    2. Xiaohui Wu, Guoli Ji, Qingshun Quinn Li
      Pages 3-11
    3. Xiaohui Wu, Guoli Ji, Qingshun Quinn Li
      Pages 13-23
    4. Min Dong, Guoli Ji, Qingshun Quinn Li, Chun Liang
      Pages 25-37
    5. Xiaohui Wu, Guoli Ji, Qingshun Quinn Li
      Pages 39-48
  3. Biochemistry of Polyadenylation in Plants

  4. Genome-Scale Study of Polyadenylation in Plants

    1. Front Matter
      Pages 145-145
    2. Balasubrahmanyam Addepalli, Suryadevara Rao, Arthur G. Hunt
      Pages 147-158
    3. Pratap Kumar Pati, Liuyin Ma, Arthur G. Hunt
      Pages 159-174
    4. Jingyi Cao, Qingshun Quinn Li
      Pages 185-194
  5. Back Matter
    Pages 223-225

About this book


This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. Numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Some are specific for plant research, but most can be adopted for research in other organisms.

Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3'-end formation in plants.


bioinformatics cytoplasm eukaryotes gene expression mRNA mRNA-poly(A) junction on a genome-wide scale plant polyadenylation signals polyadenylation protein interacting partners sequence tags

Editors and affiliations

  • Arthur G. Hunt
    • 1
  • Qingshun Quinn Li
    • 2
  1. 1.Department of Plant and Soil SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Department of Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and EcologyMiami University, Oxford, OH, USA and Xiamen UniversityXiamen, FujianChina

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2174-4
  • Online ISBN 978-1-4939-2175-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site