Clinical Proteomics

Methods and Protocols

  • Antonia Vlahou
  • Manousos Makridakis

Part of the Methods in Molecular Biology book series (MIMB, volume 1243)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Sample Collection

    1. Front Matter
      Pages 1-1
    2. Vasiliki Lygirou, Manousos Makridakis, Antonia Vlahou
      Pages 3-27
    3. Agnieszka Latosinska, Maria Frantzi, William Mullen, Antonia Vlahou, Manousos Makridakis
      Pages 29-41
    4. Marina Pitto, Samuele Corbetta, Francesca Raimondo
      Pages 43-53
    5. Marta Cova, Rui Oliveira-Silva, José Alexandre Ferreira, Rita Ferreira, Francisco Amado, Ana Luísa Daniel-da-Silva et al.
      Pages 83-100
  3. Technologies for Discovery and Validation

    1. Front Matter
      Pages 101-101
    2. Laura Santucci, Maurizio Bruschi, Gian Marco Ghiggeri, Giovanni Candiano
      Pages 103-125
    3. Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, Giovanni Candiano
      Pages 127-138
    4. Veronica Mainini, Maciej Lalowski, Athanasios Gotsopoulos, Vasiliki Bitsika, Marc Baumann, Fulvio Magni
      Pages 139-164
    5. Magali Court, Jérôme Garin, Christophe D. Masselon
      Pages 175-186
    6. Angelique Stalmach, Holger Husi, Khedidja Mosbahi, Amaya Albalat, William Mullen, Harald Mischak
      Pages 187-205
  4. Extracting Biological Relevant Biomarkers and Proceeding with Implementation

    1. Front Matter
      Pages 221-221
    2. Akshay Bhat, Mohammed Dakna, Harald Mischak
      Pages 237-253
    3. Efthymios Manolis, Armin Koch, Dieter Deforce, Spiros Vamvakas
      Pages 255-272
  5. Back Matter
    Pages 273-278

About this book


This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents  approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators working on biomarker development.


mass spectrometry-based approaches omics datasets protein protein functional protein identification protein post-translational protein quantification proteomic biomarker

Editors and affiliations

  • Antonia Vlahou
    • 1
  • Manousos Makridakis
    • 2
  1. 1.Biomedical Research FoundationAcademy of AthensAthensGreece
  2. 2.Biomedical Research FoundationAcademy of AthensAthensGreece

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1871-3
  • Online ISBN 978-1-4939-1872-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site