© 2015

Nuclear G-Protein Coupled Receptors

Methods and Protocols

  • Bruce G. Allen
  • Terence E. Hébert

Part of the Methods in Molecular Biology book series (MIMB, volume 1234)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Richard N. Re, Julia L. Cook
    Pages 1-8
  3. David Chatenet, Steve Bourgault, Alain Fournier
    Pages 17-30
  4. Clémence Merlen, Louis R. Villeneuve, Bruce G. Allen
    Pages 31-41
  5. Erika F. Dahl, Casey D. Wright, Timothy D. O’Connell
    Pages 43-52
  6. Artavazd Tadevosyan, Bruce G. Allen, Stanley Nattel
    Pages 69-80
  7. Vikrant K. Bhosle, Fernand Gobeil Jr., Jose Carlos Rivera, Alfredo Ribeiro-da-Silva, Sylvain Chemtob
    Pages 81-97
  8. Ying-Nai Wang, Longfei Huo, Jennifer L. Hsu, Mien-Chie Hung
    Pages 99-112
  9. Senka Ljubojević, Donald M. Bers
    Pages 135-147
  10. Rhiannon Campden, Darlaine Pétrin, Mélanie Robitaille, Nicolas Audet, Sarah Gora, Stéphane Angers et al.
    Pages 161-184
  11. George Vaniotis, Sarah Gora, André Nantel, Terence E. Hébert, Bruce G. Allen
    Pages 185-195
  12. Davide Calebiro, Amod Godbole, Sandra Lyga, Martin J. Lohse
    Pages 197-211
  13. Back Matter
    Pages 213-215

About this book


Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.


G protein signaling GPCRs intacrine signaling systems nuclear GPCRs nuclear membrane receptors

Editors and affiliations

  • Bruce G. Allen
    • 1
  • Terence E. Hébert
    • 2
  1. 1.Montreal Heart Institute & Université de MontréalMontrealCanada
  2. 2.Pharmacology and TherapeuticsMcGill UniversityMontrealCanada

Bibliographic information

  • Book Title Nuclear G-Protein Coupled Receptors
  • Book Subtitle Methods and Protocols
  • Editors Bruce G. Allen
    Terence E. Hébert
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-1754-9
  • Softcover ISBN 978-1-4939-5546-6
  • eBook ISBN 978-1-4939-1755-6
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XV, 215
  • Number of Illustrations 28 b/w illustrations, 15 illustrations in colour
  • Topics Protein Science
  • Buy this book on publisher's site