Opioid Receptors

Methods and Protocols

  • Santi M. Spampinato

Part of the Methods in Molecular Biology book series (MIMB, volume 1230)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Genetic, Structural Biology, Transcriptional and Post-transcriptional Analysis of Opioid Receptors

    1. Front Matter
      Pages 1-1
    2. Cheol Kyu Hwang, Yadav Wagley, Ping-Yee Law, Li-Na Wei, Horace H. Loh
      Pages 39-51
    3. Michal Korostynski, Marcin Piechota, Slawomir Golda, Ryszard Przewlocki
      Pages 65-76
  3. Cellular Detection and Analysis of Opioid Receptors

  4. Analysis of Signaling Events Modulated by Opioid Receptors

    1. Front Matter
      Pages 167-167
    2. Alfhild Grönbladh, Mathias Hallberg
      Pages 169-176
    3. Viola Spahn, Dinah Nockemann, Halina Machelska
      Pages 187-196
    4. Viola Spahn, Dinah Nockemann, Halina Machelska
      Pages 197-211
  5. Model Systems to Studying Opioid Receptor-Mediated Functions

    1. Front Matter
      Pages 213-213
    2. María Rodríguez-Muñoz, Pilar Sánchez-Blázquez, Concha Bailón, Javier Garzón
      Pages 233-241
    3. Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, Paola Sacerdote
      Pages 253-261
    4. Mara Castelli, Alberto Panerai, Paola Sacerdote, Silvia Franchi
      Pages 263-271
  6. Behavioral Effects Mediated by Opioid Receptors

    1. Front Matter
      Pages 279-279
    2. Liana Fattore, Paola Fadda, Silvia Antinori, Walter Fratta
      Pages 281-293
    3. Liana Fattore, Paola Fadda, Mary Tresa Zanda, Walter Fratta
      Pages 295-307
  7. Back Matter
    Pages 323-326

About this book


Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.


Agonist ligands Behavioral analysis Cell signaling modulation Cellular detection Computational analysis Immunodetection Opioid receptor genetics Opioid-mediated seeking behavior Post-transcriptional regulation

Editors and affiliations

  • Santi M. Spampinato
    • 1
  1. 1.University of BolognaBolognaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1707-5
  • Online ISBN 978-1-4939-1708-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site