Agrobacterium Protocols

Volume 1

  • Kan Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 1223)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Vectors and Model Plants

    1. Front Matter
      Pages 1-1
    2. Mark A. Smedley, Wendy A. Harwood
      Pages 3-16
    3. Jennifer N. Bragg, Amy Anderton, Rita Nieu, John P. Vogel
      Pages 17-33
    4. Viviane Cosson, Alexis Eschstruth, Pascal Ratet
      Pages 43-56
    5. Joyce Van Eck, Kerry Swartwood
      Pages 57-67
  3. Cereal Plants

    1. Front Matter
      Pages 69-69
    2. Cornelia Marthe, Jochen Kumlehn, Goetz Hensel
      Pages 71-83
    3. Ingrid Otto, Andrea Müller, Jochen Kumlehn
      Pages 85-99
    4. Bronwyn Frame, Katey Warnberg, Marcy Main, Kan Wang
      Pages 101-117
    5. Shireen K. Assem
      Pages 119-134
    6. Stanislaus Antony Ceasar, Savarimuthu Ignacimuthu
      Pages 135-142
    7. Sebastian Gasparis, Anna Nadolska-Orczyk
      Pages 143-153
    8. Yukoh Hiei, Yuji Ishida, Toshihiko Komari
      Pages 155-167
    9. Marcy Main, Bronwyn Frame, Kan Wang
      Pages 169-180
    10. Xiaomei Guo, Zhengxiang Ge, Shirley J. Sato, Tom E. Clemente
      Pages 181-188
    11. Yuji Ishida, Masako Tsunashima, Yukoh Hiei, Toshihiko Komari
      Pages 189-198
    12. Eva Medvecká, Wendy A. Harwood
      Pages 199-209
  4. Legume Plants

    1. Front Matter
      Pages 211-211
    2. Chunxiang Fu, Timothy Hernandez, Chuanen Zhou, Zeng-Yu Wang
      Pages 213-221
    3. Samira Rahimi-Ashtiani, Sareena Sahab, Stephen Panter, John Mason, German Spangenberg
      Pages 223-235
    4. Michael L. Sullivan, Kenneth H. Quesenberry
      Pages 237-254
    5. Ratikanta Behura, Sanjeev Kumar, Bedabrata Saha, Manasa Kumar Panda, Mohitosh Dey, Ayan Sadhukhan et al.
      Pages 255-264
    6. Ufuk Celikkol Akcay, Meral Yücel, Hüseyin Avni Öktem
      Pages 265-274
    7. Diane Luth, Katey Warnberg, Kan Wang
      Pages 275-284
  5. Vegetable Plants

    1. Front Matter
      Pages 285-285
    2. Penny A. C. Hundleby née Sparrow, Judith A. Irwin
      Pages 287-297
    3. Jung Min, Sun Hee Shin, En Mi Jeon, Jung Mi Park, Ji Young Hyun, Chee Hark Harn
      Pages 311-320
    4. Iris Heidmann, Kim Boutilier
      Pages 321-334
    5. Hiroyo Kagami, Masayuki Kurata, Hiroaki Matsuhira, Kazunori Taguchi, Tetsuo Mikami, Hideto Tamagake et al.
      Pages 335-347
    6. Dora Garcia, Javier Narváez-Vásquez, Martha L. Orozco-Cárdenas
      Pages 349-361
  6. Hiroyo Kagami, Masayuki Kurata, Hiroaki Matsuhira, Kazunori Taguchi, Tetsuo Mikami, Hideto Tamagake et al.
    Pages E1-E1
  7. Back Matter
    Pages 363-368

About this book


Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.

  Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and  three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis.  It also updates a chapter for vector construction, a step critical to a successful plant transformation process.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition  facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.


Agrobacterium-mediated transformation DNA molecules agricultural crops barley biolistic gun citrus electroporation integrated transgenes plant cells plant materials plant species polyethylene glycol rice wheat

Editors and affiliations

  • Kan Wang
    • 1
  1. 1.Plant Sciences Institute, and Department of AgronomyIowa State University, Center for Plant TransformationAmesUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1694-8
  • Online ISBN 978-1-4939-1695-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site