Stem Cells and Tissue Repair

Methods and Protocols

  • Chrissa Kioussi

Part of the Methods in Molecular Biology book series (MIMB, volume 1210)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Axel P. Gross, Chrissa Kioussi
    Pages 1-8
  3. Ana Alastrue-Agudo, Slaven Erceg, Marta Cases-Villar, Viviana Bisbal-Velasco, Richard J. Griffeth, Francisco Javier Rodriguez-Jiménez et al.
    Pages 23-35
  4. Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora et al.
    Pages 37-49
  5. Qiuyue Liu, Andrzej Swistowski, Xianmin Zeng
    Pages 79-90
  6. Federico Ferro, Renza Spelat, Chelsea S. Baheney
    Pages 91-115
  7. Adriana Di Benedetto, Claudia Carbone, Giorgio Mori
    Pages 117-130
  8. Ran-Ran Zhang, Takanori Takebe, Leina Miyazaki, Maho Takayama, Hiroyuki Koike, Masaki Kimura et al.
    Pages 131-141
  9. Tomoyuki Yamaguchi, Sanae Hamanaka, Hiromitsu Nakauchi
    Pages 143-150
  10. Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok et al.
    Pages 161-181
  11. Marie Schneider, Christina Dieckmann, Katrin Rabe, Jan-Christoph Simon, Vuk Savkovic
    Pages 203-227
  12. Back Matter
    Pages 249-250

About this book


Stem Cells and Tissue Repair: Methods and Protocols presents in-depth methods for the three major approaches of rejuvenating an aging or sick body: latent regenerative capacity stimulated in a targeted way, replacement organs grown de novo and surgically implanted, and tissue surgically implanted and coaxed to integrate and restore problem areas. The first approach taps into the latent regenerative capacity of particular tissues, such as muscle, skin, fat, or bone marrow. The second approach induces and grows pluripotent stem cells, drives their differentiation along certain pathways such as germ layers, neural crest, liver, teeth or retina, and cultures organs such as pancreas and heart. While the third approach involves various engraftment techniques for neural tissue. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Stem Cells and Tissue Repair: Methods and Protocols provides state-of the-art methods descriptions and the references therein that will provide a suitable starting point for exploring the vast literature that has already developed for regenerative medicine.


mouse embryonic stem cells neural stem cells pluripotent stem cells stem cells tissue engineering

Editors and affiliations

  • Chrissa Kioussi
    • 1
  1. 1.Department of Pharmaceutical SciencesCollege of Pharmacy, Oregon State UniversityCorvallisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1434-0
  • Online ISBN 978-1-4939-1435-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site