T-Helper Cells

Methods and Protocols

  • Ari Waisman
  • Burkhard Becher

Part of the Methods in Molecular Biology book series (MIMB, volume 1193)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Isolation of T Cells from the Tissues

    1. Front Matter
      Pages 1-1
    2. Beate Lorenz, Esther von Stebut
      Pages 3-13
    3. Iva Lelios, Melanie Greter
      Pages 15-19
    4. Sonja Reißig, Christopher Hackenbruch, Nadine Hövelmeyer
      Pages 21-25
    5. Isis Ludwig-Portugall, Christian Kurts
      Pages 27-35
  3. Analysis of T Cell Function and Phenotypes

    1. Front Matter
      Pages 37-37
    2. Florian Mair, Vinko Tosevski
      Pages 39-49
    3. Vinko Tosevski, Florian Mair
      Pages 51-54
    4. Oliver Gorka, Stefan Wanninger, Jürgen Ruland
      Pages 69-83
    5. Christian Thomas Mayer, Tim Sparwasser
      Pages 85-96
    6. Julia M. Braun, Christina E. Zielinski
      Pages 97-104
    7. Julie Rumble, Benjamin M. Segal
      Pages 105-113
  4. In Vivo Models of T Cell Function

    1. Front Matter
      Pages 115-115
    2. Elisa Kieback, Ellen Hilgenberg, Simon Fillatreau
      Pages 117-126
    3. Helen Meyer-Martin, Sebastian Reuter, Christian Taube
      Pages 127-141
    4. Anna Śledzińska, Lynsey Fairbairn, Thorsten Buch
      Pages 153-169

About this book

Introduction

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo, and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

Keywords

T cells T-helper cells gene targeting mouse models phenotypes

Editors and affiliations

  • Ari Waisman
    • 1
  • Burkhard Becher
    • 2
  1. 1.Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of MainzMainzGermany
  2. 2.University of Zurich Institute of Experimental ImmunologyZurichGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1212-4
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1211-7
  • Online ISBN 978-1-4939-1212-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book