Basophils and Mast Cells

Methods and Protocols

  • Bernhard F. Gibbs
  • Franco H. Falcone

Part of the Methods in Molecular Biology book series (MIMB, volume 1192)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction & Historical Background

  3. Obtaining the Cells: Purification, Culture, Counting, and Cell Lines

    1. Front Matter
      Pages 33-33
    2. Franco H. Falcone, Bernhard F. Gibbs
      Pages 35-47
    3. Jasmine Farrington, Elizabeth P. Seward, Peter T. Peachell
      Pages 49-55
    4. Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, Metin Artuc
      Pages 69-85
    5. Elena Borzova, Clemens A. Dahinden
      Pages 87-100
  4. Whole Cells: Identification in Tissues, Signaling Pathways, Gene Silencing, and Reporters

    1. Front Matter
      Pages 115-115
    2. Andrew F. Walls, Cornelia Amalinei
      Pages 117-134
    3. Bettina M. Jensen, Sidsel Falkencrone, Per S. Skov
      Pages 135-145
    4. Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, Didier G. Ebo
      Pages 147-159
    5. Monika Bambousková, Zuzana Hájková, Pavel Dráber, Petr Dráber
      Pages 161-176
    6. Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J. C. Alcocer, Franco H. Falcone
      Pages 177-184
    7. Vadim V. Sumbayev, Bernhard F. Gibbs
      Pages 185-191
    8. Edward F. Knol, Bernhard F. Gibbs
      Pages 193-203
    9. José Mário Morgado, Laura Sánchez-Muñoz, Cristina Teodósio, Luís Escribano
      Pages 205-226
  5. Whole Organism: Disease and Knockout Models

    1. Front Matter
      Pages 227-227
    2. Christian Schwartz, David Voehringer
      Pages 229-237
    3. Vladimir Andrey Giménez-Rivera, Martin Metz, Frank Siebenhaar
      Pages 239-247
  6. Back Matter
    Pages 249-251

About this book


In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most.

Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.


Allergic inflammation Anti-allergic therapies Basophil purification Immune system Mast cell functions Signaling pathways

Editors and affiliations

  • Bernhard F. Gibbs
    • 1
  • Franco H. Falcone
    • 2
  1. 1.Medway School of PharmacyThe University of KentChatham MaritimeUnited Kingdom
  2. 2.Division of Molecular and Cellular Science, The School of PharmacyThe University of NottinghamNottinghamUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1172-1
  • Online ISBN 978-1-4939-1173-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site