Metabolic Flux Analysis

Methods and Protocols

  • Jens O. Krömer
  • Lars K. Nielsen
  • Lars M. Blank

Part of the Methods in Molecular Biology book series (MIMB, volume 1191)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Models: Stoichiometry, Network Generation

    1. Front Matter
      Pages 1-1
    2. Ric Colasanti, Janaka N. Edirisinghe, Tahmineh Khazaei, José P. Faria, Sam Seaver, Fangfang Xia et al.
      Pages 19-45
  3. Thermodynamics: A Step Beyond MFA

    1. Front Matter
      Pages 47-47
  4. Experiments: Design Considerations, Fermentation, Analytics

    1. Front Matter
      Pages 79-79
    2. Lake-Ee Quek, Lars K. Nielsen
      Pages 81-90
    3. S. Aljoscha Wahl, Reza Maleki Seifar, Angela ten Pierick, Cor Ras, Jan C. van Dam, Joseph J. Heijnen et al.
      Pages 91-105
    4. Jabin Watson, Bernard Degnan, Sandie Degnan, Jens O. Krömer
      Pages 107-125
    5. Birgitta E. Ebert, Lars M. Blank
      Pages 127-142
    6. Paula Jouhten, Hannu Maaheimo
      Pages 143-164
    7. Judith Becker, Christoph Wittmann
      Pages 165-174
  5. 13C Fluxomics Data Processing

    1. Front Matter
      Pages 195-195
    2. Pierre Millard, Fabien Letisse, Serguei Sokol, Jean-Charles Portais
      Pages 197-207
    3. Lake-Ee Quek, Lars K. Nielsen
      Pages 209-224
    4. Christian Krach, Astrid Junker, Hendrik Rohn, Falk Schreiber, Björn H. Junker
      Pages 225-233
  6. Examples

    1. Front Matter
      Pages 235-235
    2. Yu Matsuoka, Kazuyuki Shimizu
      Pages 237-260
    3. Yu Matsuoka, Kazuyuki Shimizu
      Pages 261-289
  7. Back Matter
    Pages 315-316

About this book


Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from 13C experiments, and three chapters that summarize some recent key findings. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Metabolic Flux Analysis: Methods and Protocols presents protocols that cover a range of relevant organisms currently used in the field, providing a solid basis to anybody interested in the field of metabolic flux analysis.


cell culture flux analysis metabolomics stoichiometry thermodynamics

Editors and affiliations

  • Jens O. Krömer
    • 1
  • Lars K. Nielsen
    • 2
  • Lars M. Blank
    • 3
  1. 1.Centre for Microbial Electrosynthesis (CEMES), Advanced Water Management CentreUniversity of QueenslandSt. Lucia, BrisbaneAustralia
  2. 2.AIBNUniversity of QueenslandSt. Lucia, BrisbaneAustralia
  3. 3.Biology DepartmentRWTH Aachen UniversityAachenGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1169-1
  • Online ISBN 978-1-4939-1170-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site