Hematopoietic Stem Cell Protocols

  • Kevin D. Bunting
  • Cheng-Kui Qu

Part of the Methods in Molecular Biology book series (MIMB, volume 1185)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Kevin D. Bunting, Cheng-Kui Qu
      Pages 3-6
  3. Stem Cell Enrichment and Heterogeneity

  4. Molecular and Cellular Characterization

    1. Front Matter
      Pages 89-89
    2. Wenhuo Hu, Christopher Y. Park
      Pages 121-140
    3. Amber Hogart Begtrup
      Pages 141-153
    4. Fatih Kocabas, Junke Zheng, Chengcheng Zhang, Hesham A. Sadek
      Pages 155-164
    5. Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, Kevin D. Bunting
      Pages 165-177
  5. In Vitro Assays and Differentiation

    1. Front Matter
      Pages 179-179
    2. Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, Deborah L. French
      Pages 181-194
    3. Phuong L. Doan, John P. Chute
      Pages 211-221
    4. Byungwook Ahn, Zhengqi Wang, David R. Archer, Wilbur A. Lam
      Pages 223-233
  6. Transplantation Assays and Imaging Engraftment

    1. Front Matter
      Pages 235-235
    2. Ryo Yamamoto, Yohei Morita, Hiromitsu Nakauchi
      Pages 237-245
    3. Juwell W. Wu, Judith M. Runnels, Charles P. Lin
      Pages 247-265
    4. Ken-Edwin Aryee, Leonard D. Shultz, Michael A. Brehm
      Pages 267-278
    5. Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, Linheng Li
      Pages 279-284
  7. Genetic Modification

    1. Front Matter
      Pages 285-285
    2. Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, David A. Williams
      Pages 287-309
    3. Niek P. van Til, Gerard Wagemaker
      Pages 311-319
    4. Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, Hans-Peter Kiem
      Pages 321-344
    5. Leonid V. Bystrykh, Gerald de Haan, Evgenia Verovskaya
      Pages 345-360
  8. Back Matter
    Pages 361-364

About this book


Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases.  An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation.


gene expression gene profiling hematopoietic stem cells heterogeneity in vitro assays

Editors and affiliations

  • Kevin D. Bunting
    • 1
  • Cheng-Kui Qu
    • 2
  1. 1.Department of PediatricsAflac Cancer and Blood Disorders Center Emory University School of MedicineAtlantaUSA
  2. 2.Department of PediatricsEmory University School of Medicine Aflac Cancer & Blood Disorders CtrAtlantaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1132-5
  • Online ISBN 978-1-4939-1133-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site