Animal Endo-SiRNAs

Methods and Protocols

  • Andreas Werner

Part of the Methods in Molecular Biology book series (MIMB, volume 1173)

Table of contents

  1. Front Matter
    Pages i-x
  2. Sammer Alnumeir, Andreas Werner
    Pages 27-32
  3. Katharina Elmer, Stephanie Helfer, Milijana Mirkovic-Hösle, Klaus Förstemann
    Pages 33-49
  4. Do-Hwan Lim, Chun-Taek Oh, Sung-Jun Han, Young Sik Lee
    Pages 51-58
  5. Philip K. Shiu, Jimmy J. Zhuang, Craig P. Hunter
    Pages 71-87
  6. Mark Carlile, Andreas Werner
    Pages 89-98
  7. Jingmin Jin, Larry A. McReynolds, Monika Gullerova
    Pages 99-111
  8. Teresa T. Liu, Zhihua Li, Bino John
    Pages 123-133
  9. Christophe Antoniewski
    Pages 135-146
  10. Marius van den Beek, Christophe Antoniewski, Clément Carré
    Pages 147-155
  11. Simon P. Keam, Andrew Sobala, David T. Humphreys, Catherine M. Suter, Gyorgy Hutvagner
    Pages 157-167
  12. Back Matter
    Pages 181-182

About this book

Introduction

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs.  These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.

 

Keywords

C. elegans Dicer molecule Short interfering RNAs (siRNAs) endo-siRNAs gene expression model systems short RNAs small RNAs vertebrates

Editors and affiliations

  • Andreas Werner
    • 1
  1. 1.Institute for Cell and Molecular BioscieNewcastle UniversityUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0931-5
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0930-8
  • Online ISBN 978-1-4939-0931-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book