Quantitative Real-Time PCR

Methods and Protocols

  • Roberto Biassoni
  • Alessandro Raso

Part of the Methods in Molecular Biology book series (MIMB, volume 1160)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Alessandro Raso, Roberto Biassoni
    Pages 1-3
  3. Gemma Johnson, Afif Abdel Nour, Tania Nolan, Jim Huggett, Stephen Bustin
    Pages 5-17
  4. Jan Hellemans, Jo Vandesompele
    Pages 19-26
  5. Francisco Bizouarn
    Pages 27-41
  6. Benedikt Kirchner, Vijay Paul, Irmgard Riedmaier, Michael W. Pfaffl
    Pages 43-53
  7. Simon Wadle, Stefanie Rubenwolf, Michael Lehnert, Bernd Faltin, Manfred Weidmann, Frank Hufert et al.
    Pages 55-73
  8. Domenico Russo, Mauro Severo Malnati
    Pages 75-86
  9. Pierre Foskett, Gareth Gerrard, Letizia Foroni
    Pages 115-124
  10. Pamela Pinzani, Francesca Salvianti, Claudio Orlando, Mario Pazzagli
    Pages 133-145
  11. Michael Eikmans, Jacqueline D. H. Anholts, Frans H. J. Claas
    Pages 147-163
  12. Swanhild U. Meyer, Katharina Stoecker, Steffen Sass, Fabian J. Theis, Michael W. Pfaffl
    Pages 165-188
  13. Francisco Bizouarn
    Pages 189-214
  14. Back Matter
    Pages 229-231

About this book


Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.


Digital PCR Microbiological detections Nucleic acid measurement Pathological applications Quality issues Translational medicine applications qPCR

Editors and affiliations

  • Roberto Biassoni
    • 1
  • Alessandro Raso
    • 2
  1. 1.Laboratorio Medicina Molecolare, Dipartimento Medicina TraslazionaleIstituto Giannina GasliniGenovaItaly
  2. 2.Laboratorio U.O.C NeurochirurgiaIstituto Giannina GasliniGenovaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0732-8
  • Online ISBN 978-1-4939-0733-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site