Photoswitching Proteins

Methods and Protocols

  • Sidney Cambridge

Part of the Methods in Molecular Biology book series (MIMB, volume 1148)

Table of contents

  1. Front Matter
    Pages i-x
  2. Matthew K. Daddysman, Michael A. Tycon, Christopher J. Fecko
    Pages 1-17
  3. Andrei V. Karginov, Klaus M. Hahn, Alexander Deiters
    Pages 31-43
  4. Andreas Reiner, Ehud Y. Isacoff
    Pages 45-68
  5. Martin Sumser, Philipp Stawski
    Pages 69-76
  6. Lauren R. Polstein, Charles A. Gersbach
    Pages 89-107
  7. Consuelo Fois, Pierre-Hugues Prouvot, Albrecht Stroh
    Pages 129-147
  8. Marion Mutter, Natalia Swietek, Thomas A. Münch
    Pages 149-160
  9. Wagner Steuer Costa, Jana Liewald, Alexander Gottschalk
    Pages 161-175
  10. Chenxi Duan, Virgile Adam, Martin Byrdin, Dominique Bourgeois
    Pages 177-202
  11. Archan Ganguly, Subhojit Roy
    Pages 203-215
  12. William P. Dempsey, Hanyu Qin, Periklis Pantazis
    Pages 217-228
  13. Yuji Ishitsuka, Karin Nienhaus, G. Ulrich Nienhaus
    Pages 239-260
  14. Benjamien Moeyaert, Peter Dedecker
    Pages 261-276
  15. Back Matter
    Pages 277-278

About this book

Introduction

Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Keywords

fluorescent proteins light light-dependent light-responsive proteins neurobiology neuronal tissue opsin based channels optical photoswitching proteins proteins

Editors and affiliations

  • Sidney Cambridge
    • 1
  1. 1.Institute of Anatomy and Cell BiologyUniversity of HeidelbergHeidelbergGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0470-9
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0469-3
  • Online ISBN 978-1-4939-0470-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book