Microbial Biofilms

Methods and Protocols

  • Gianfranco Donelli

Part of the Methods in Molecular Biology book series (MIMB, volume 1147)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Investigations on Biofilms in Health and Disease

    1. Front Matter
      Pages 1-1
    2. Claus Sternberg, Thomas Bjarnsholt, Mark Shirtliff
      Pages 3-22
    3. Mohammed Dwidar, Shuichi Takayama, Robert J. Mitchell
      Pages 23-32
    4. Marc M. Baum, Manjula Gunawardana, Paul Webster
      Pages 85-103
    5. Laura Nistico, Luanne Hall-Stoodley, Paul Stoodley
      Pages 105-126
    6. Kim Thomsen, Hannah Trøstrup, Claus Moser
      Pages 127-139
    7. Jyotsna Chandra, Eric Pearlman, Mahmoud A. Ghannoum
      Pages 141-157
    8. Marios Arvanitis, Beth Burgwyn Fuchs, Eleftherios Mylonakis
      Pages 159-172
  3. Investigations on Anti-biofilm Compounds and Strategies

  4. Investigations on Biofilms in the Environment and Manufacturing Plants

  5. Back Matter
    Pages 379-380

About this book


The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms.


assay protocols bacterial biofilms bacterial communities biofilm matrix fungal biofilms

Editors and affiliations

  • Gianfranco Donelli
    • 1
  1. 1.Fondazione Santa Lucia IRCCS Microbial Biofilm LaboratoryRomeItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0466-2
  • Online ISBN 978-1-4939-0467-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site