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Part of the Methods in Molecular Biology book series (MIMB, volume 1142)
Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.
From the book reviews:
“This book offers insights and protocols regarding 16 assays used by scientists studying the workings of the immune system, particularly with respect to autoimmunity. … It is intended for scientists who are interested in reviewing the theory behind a technique and/or tips for conducting it. Clinicians trying to gain a better understanding of scientific techniques as they are appraising the primary literature in their field also could presumably use it.” (Tiphanie Vogel, Doody’s Book Reviews, September, 2014)