Arthritis Research

Methods and Protocols

  • Shunichi Shiozawa

Part of the Methods in Molecular Biology book series (MIMB, volume 1142)

Table of contents

  1. Front Matter
    Pages i-x
  2. Shingo Eikawa, Shusaku Mizukami, Heiichiro Udono
    Pages 11-17
  3. Yumi Miyazaki, Shunichi Shiozawa
    Pages 49-53
  4. Ghada Alsaleh, Jacques-Eric Gottenberg
    Pages 55-63
  5. Asako Chiba, Sachiko Miyake
    Pages 75-84
  6. Masaomi Obata, Mareki Ohtsuji, Yukiyasu Iida, Toshikazu Shirai, Sachiko Hirose, Hioroyuki Nishimura
    Pages 111-141
  7. Gábor Hullám, András Gézsi, András Millinghoffer, Péter Sárközy, Bence Bolgár, Sanjeev K. Srivastava et al.
    Pages 143-176
  8. Antti Isomäki, Tarvo Sillat, Mari Ainola, Mikko Liljeström, Yrjö T. Konttinen, Mika Hukkanen
    Pages 189-201
  9. Back Matter
    Pages 203-204

About this book


Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.


autoimmune disease microRNAs mouse models rheumatoid arthritis transgene techniques

Editors and affiliations

  • Shunichi Shiozawa
    • 1
  1. 1.Department of RheumatologyKyushu University Beppu HospitalBeppuJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0403-7
  • Online ISBN 978-1-4939-0404-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site