Molecular Interactions in Bioseparations

  • That T. Ngo

Table of contents

  1. Front Matter
    Pages i-xvii
  2. General Introduction

    1. Front Matter
      Pages 1-1
    2. Indu Parikh, Pedro Cuatrecasas
      Pages 3-13
    3. Sten Ohlson, David Zopf
      Pages 15-25
    4. Dyer Narinesingh, That T. Ngo
      Pages 49-68
    5. Jun Hirabayashi, Ken-ichi Kasai
      Pages 69-87
  3. Affinity Chromatography with Biological Ligands

    1. Front Matter
      Pages 89-89
    2. Michael D. P. Boyle, Ervin L. Faulmann, Dennis W. Metzger
      Pages 91-112
    3. Tsutomu Tsuji, Kazuo Yamamoto, Toshiaki Osawa
      Pages 113-126
    4. Shin-ichi Ishii, Takashi Kumazaki
      Pages 127-138
    5. Michele Nachman-Clewner, Cheryl Spence, Pascal Bailon
      Pages 139-149
    6. Francisco M. Alvarez, Carey B. Bottom, Prashant Chikhale, Charles Pidgeon
      Pages 151-167
    7. Karen L. Williams, Apryll M. Stalcup
      Pages 189-202
  4. Immunoaffinity Separation

    1. Front Matter
      Pages 203-203
    2. Peter J. Richardson, J. Paul Luzio
      Pages 213-227
    3. John Ugelstad, Ørjan Olsvik, Ruth Schmid, Arvid Berge, Steinar Funderud, Kjell Nustad
      Pages 229-244
  5. Affinity Chromatography with Biomimetic Ligands

    1. Front Matter
      Pages 245-245
    2. Earle Stellwagen
      Pages 247-255
    3. M. A. Vijayalakshmi
      Pages 257-275
    4. T. William Hutchens, Tai-Tung Yip
      Pages 277-312
    5. Milan J. Beneš, Alexandra Štambergova, William H. Scouten
      Pages 313-322
    6. Tetsuya Tosa, Tadashi Sato, Taizo Watanabe, Satoshi Minobe
      Pages 323-332
    7. Patrick Hubert, Edith Dellacherie
      Pages 333-359
  6. Novel Concepts and Applications

    1. Front Matter
      Pages 361-361
    2. Lars I. Andersson, Björn Ekberg, Klaus Mosbach
      Pages 383-394
    3. Bo Mattiasson, Eva Linné, Rajni Kaul
      Pages 395-401
    4. S. Oscarsson, J. Porath
      Pages 403-413
    5. That T. Ngo, Neeta Khatter
      Pages 415-430
  7. Affinity-Related Techniques

    1. Front Matter
      Pages 431-431
    2. Bo Mattiasson, Rajni Kaul
      Pages 469-477
    3. Mathias Uhlén, Ørjan Olsvik, Erik Hornes
      Pages 479-485
    4. Rajni Kaul, Bo Mattiasson
      Pages 487-498
    5. Gerhard Kopperschläger, Gerd Birkenmeier
      Pages 499-509
    6. Gabor L. Igloi
      Pages 511-531
    7. Matthijs Dekker
      Pages 533-544
  8. Back Matter
    Pages 545-570

About this book


Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica­ tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.


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Editors and affiliations

  • That T. Ngo
    • 1
  1. 1.Department of Developmental and Cell BiologyUniversity of CaliforniaIrvineUSA

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