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Protein Analysis and Purification

Benchtop Techniques

  • Ian M. Rosenberg

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Ian M. Rosenberg
    Pages 1-4
  3. Ian M. Rosenberg
    Pages 5-18
  4. Ian M. Rosenberg
    Pages 19-54
  5. Ian M. Rosenberg
    Pages 55-98
  6. Ian M. Rosenberg
    Pages 99-133
  7. Ian M. Rosenberg
    Pages 135-152
  8. Ian M. Rosenberg
    Pages 183-206
  9. Ian M. Rosenberg
    Pages 207-264
  10. Ian M. Rosenberg
    Pages 265-324
  11. Ian M. Rosenberg
    Pages 325-363
  12. Back Matter
    Pages 365-434

About this book

Introduction

This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde­ pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi­ nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip­ ment other than encouragement to read the manual, if you can find it. Through­ out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.

Keywords

DNA Nucleotide amino acid boundary element method chemistry design e-Book enzyme enzymes form nucleic acid object protein review sequencing

Authors and affiliations

  • Ian M. Rosenberg
    • 1
  1. 1.Massachusetts General HospitalBostonUSA

Bibliographic information