Immobilized Biochemicals and Affinity Chromatography

  • R. Bruce Dunlap

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Affinity Chromatography

    1. Front Matter
      Pages 1-1
    2. Steven C. March, Indu Parikh, Pedro Cuatrecasas
      Pages 3-14
    3. A. H. Nishikawa, P. Bailon, A. H. Ramel
      Pages 33-42
    4. Donald L. Eshenbaugh, Donald Sens, Eric James
      Pages 61-73
    5. Houston F. Voss, Y. Ashani, Irwin B. Wilson
      Pages 75-83
    6. P. D. G. Dean, D. B. Craven, M. J. Harvey, C. R. Lowe
      Pages 99-121
  3. Immobilized Biochemicals

    1. Front Matter
      Pages 171-171
    2. M. Charles, R. W. Coughlin, B. R. Allen, E. K. Paruchuri, F. X. Hasselberger
      Pages 213-234
    3. R. I. Leavitt, F. X. Ryan, W. P. Burgess
      Pages 259-268
    4. David L. Marshall
      Pages 345-368
  4. Back Matter
    Pages 369-377

About this book


This volume contains most of the papers presented at the Sym­ posium on Affinity Chromatography and Immobilized Biochemicals, which was held on November 7-9, 1973 in Charleston, South Carolina in conjunction with the Southeastern Regional American Chemical Society meeting. The topics of the symposium represent two new biochemical frontiers which have emerged in recent years through the ingenious development and application of solid phase biochemi­ cal technologies. Affinity chromatography involves the use of selected ligands, covalently bound to a solid support such as cel­ lulose, glass, Sepharose, or polyacrylamide and exploits the princi­ ple of biochemical recognition between the ligand in the solid phase and a selected macromolecule to facilitate the rapid and often quantitative purification of enzymes, antibodies, antigens, hormones, other proteins, etc. The area of immobilized biochemicals includes the use of coenzymes, oligo- and po1ynuc1eotides, enzymes, and multistep enzyme systems which are immobilized or entrapped in the solid phase. The goals of the symposium were the revi~w of the status of affinity chromatography and immobilized biochemicals, the presentation of new data and ideas in both areas, and the establish­ ment of a dialogue between research workers in these two evolving disciplines which are so closely interrelated. The papers published in this volume provide the reader with reviews of several topics inherent in the solid phase biochemistry together with a series of timely manuscripts concerning new techniques and applications both in the use of affinity chromatography and in the investigation of immobilized biochemicals.


antibody biochemistry chemistry chromatography development enzyme enzymes macromolecule molecule paper phase protein proteins society

Editors and affiliations

  • R. Bruce Dunlap
    • 1
  1. 1.Department of ChemistryUniversity of South CarolinaColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag US 1974
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4684-6984-4
  • Online ISBN 978-1-4684-6982-0
  • Series Print ISSN 0065-2598
  • Buy this book on publisher's site