Tissue Culture of Trees

  • John H. Dodds

Table of contents

  1. Front Matter
    Pages i-xi
  2. John H. Dodds
    Pages 1-5
  3. Allan John
    Pages 6-21
  4. John H. Dodds
    Pages 22-28
  5. Teresa Bengochea
    Pages 51-55
  6. Christopher P. Wilkins, John H. Dodds
    Pages 56-79
  7. John H. Dodds
    Pages 80-87
  8. Christopher P. Wilkins, John H. Dodds
    Pages 113-136
  9. John H. Dodds
    Pages 137-137
  10. Back Matter
    Pages 138-147

About this book


1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail­ able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually described as an un­ organised proliferating mass of tissue. Although callus cultures have a great deal of potential in the biotechnological aspects of tissue culture, i.e. secondary product formation, they are not very suitable for plant propagation. The key reason for their unsuitability is that genetic aber­ rations occur during mitotic divisions in callus growth (D'amato.l965). The aberrations can be of a major type, such as aneuploidy or endo­ reduplication. It follows therefore that the genetic status of the re­ generated plants is different from that of the parent type. In general terms this genetic instability is undesirable, but there are occasions when a callus stage can be purposely included to diversify the genetic base of the crop.


Xylem development fruit growth oil palm plant plants protoplast roots science stability woody species

Editors and affiliations

  • John H. Dodds
    • 1
  1. 1.Centro de Investigación y de Estudios Avanzados del IPN.Unidad IrapuatoIrapuato, Gto.México

Bibliographic information