Immunologic Methods in Steroid Determination

  • Fernand G. Péron
  • Burton V. Caldwell

Table of contents

  1. Front Matter
    Pages i-xii
  2. Chemical and Immunologic Aspects of Antibodies to Steroids

    1. Front Matter
      Pages xv-xv
    2. David Pressman, Allan L. Grossberg
      Pages 1-14
    3. Stanley J. Gross
      Pages 41-62
  3. The Use of Antibodies for Radioimmunoassay of Steroid Hormones

    1. Front Matter
      Pages N1-N1
    2. Ian H. Thorneycroft, Stephen A. Tillson, Guy E. Abraham, Rex J. Scaramuzzi, Burton V. Caldwell
      Pages 63-86
    3. George Mikhail, Hsiu-Wu Chung, Michel Ferin, Raymond L. Vande Wiele
      Pages 113-126
    4. Stephen A. Tillson, Ian H. Thorneycroft, Guy E. Abraham, Rex J. Scaramuzzi, Burton V. Caldwell
      Pages 127-147
    5. Gordon D. Niswender, A. Rees Midgley Jr.
      Pages 149-173
  4. The Use of Antibodies to Steroids in Physiologic Studies

    1. Front Matter
      Pages N3-N3
    2. Burton V. Caldwell, Rex J. Scaramuzzi, Stephen A. Tillson, Ian H. Thorneycroft
      Pages 183-197
    3. Michel Ferin, Joseph Raziano, Antonio Tempone, Raymond L. Vande Wiele
      Pages 199-223
  5. Summary

    1. William D. Odell
      Pages 225-229
  6. Back Matter
    Pages 231-232

About this book

Introduction

The two main goals of the symposium upon which this volume is based were 1) to cement together knowledge presently available in the field of antibodies to steroids and obtainable only under separate covers in different journals and books, and 2) to present new data which could lead to a more complete understanding of physiologic phenomena like those occurring during the menstrual cycle, or to the elucidation of the mechanisms involved in steroid-protection interaction, or to the practical application of immunologic techniques to measurements of steroid hor­ mones. These techniques are extremely sensitive and can measure levels of steroid on the same order of magnitude as the radioisotope methods. However, the latter are much more laborious and costly which limits their use in many cases to the research laboratory. But the immunologic techniques generally classified as radioimmunoassay, are fraught with difficulties and problems which must be overcome. Fortunately, perhaps, the subject of immunologic techniques as applied to steroid determination is the child of radioimmunoassay of proteins, so to speak. Many of the problems which confront the former have been resolved in the latter instance. Thus, we are in an advantageous position because we are aware of the biologic and technical problems of the earlier radioimmunoassay techniques. Similar experiences have been reported in the book about the use of immunologic techniques for determination of steroid hormones.

Keywords

Laboratory antibody child hormone hormones immunology lead physiology protein proteins research steroid steroids

Editors and affiliations

  • Fernand G. Péron
    • 1
  • Burton V. Caldwell
    • 1
  1. 1.Worcester Foundation for Experimental BiologyShrewsburyUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4684-6051-3
  • Copyright Information Springer-Verlag US 1970
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4684-6053-7
  • Online ISBN 978-1-4684-6051-3
  • About this book