Nonisotopic Immunoassay

  • T. T. Ngo

Table of contents

  1. Front Matter
    Pages i-xi
  2. Enzyme-Labeled Immunoassay

    1. Front Matter
      Pages 1-1
    2. Barry J. Gould, Vincent Marks
      Pages 3-26
    3. Eiji Ishikawa, Seiichi Hashida, Takeyuki Kohno, Koichiro Tanaka
      Pages 27-55
    4. Bärbel Porstmann, Tomas Porstmann
      Pages 57-84
    5. Jean-Luc Guesdon
      Pages 85-106
    6. Y. Amir-Zaltsman, B. Gayer, E. A. Bayer, M. Wilchek, F. Kohen
      Pages 117-127
    7. Richard L. Mansell, Cecilia A. McIntosh
      Pages 147-159
  3. Luminescence Immunoassay

    1. Front Matter
      Pages 161-161
    2. Mel N. Kronick
      Pages 163-185
    3. M. P. Bailey, B. F. Rocks, C. Riley
      Pages 187-197
    4. Fukuko Watanabe, Kiyoshi Miyai
      Pages 199-209
    5. Pyare L. Khanna
      Pages 211-229
    6. Erkki Soini, Timo Lövgren
      Pages 231-243
    7. Linda B. McGown
      Pages 245-256
    8. William G. Wood, Harald Fricke, Christian J. Strasburger
      Pages 257-270
    9. F. Kohen, Y. Ausher, S. Gilad, J. De Boever, G. J. R. Barnard, J. B. Kim
      Pages 271-286
    10. Gianni Messeri, Claudio Orlando, Anna L. Caldini, Mario Pazzagli
      Pages 287-300
  4. Immunoassay at Liquid-Solid Interface

    1. Front Matter
      Pages 301-301
    2. Carl M. Berke
      Pages 303-312
    3. Hans Arwin, Stefan Welin, Ingemar Lundström
      Pages 313-330
    4. Ranald Sutherland, Claus Dähne, Rudolf Slovacek, Barry Bluestein
      Pages 331-357
  5. Membrane Immunoassay

    1. Front Matter
      Pages 359-359
    2. A. I. Vistnes
      Pages 361-388
    3. Tatsuji Yasuda, Yoshio Ishimori, Mamoru Umeda
      Pages 389-399
  6. “Particle” — Mediated Immunoassay

    1. Front Matter
      Pages 401-401
    2. William J. Litchfield
      Pages 403-413
    3. Michael Cais
      Pages 415-438
    4. Chan S. Oh, James C. Sternberg
      Pages 457-476
  7. Back Matter
    Pages 477-495

About this book


The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non­ isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.


Pet agriculture biological development environment enzyme enzymes fields infectious disease iron membrane protein proteins reaction tracer

Editors and affiliations

  • T. T. Ngo
    • 1
  1. 1.University of California, IrvineIrvineCaliforniaUSA

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