Ionic Channels in Cells and Model Systems

  • Ramon Latorre

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. Introduction

    1. Ramon Latorre
      Pages 1-2
  3. Methodologies

    1. Front Matter
      Pages 3-3
    2. Osvaldo Alvarez
      Pages 5-15
    3. Francisco Bezanilla
      Pages 37-52
    4. Brian M. Salzberg, Ana Lia Obaid, Harold Gainer
      Pages 141-162
    5. Eduardo Rojas, Rosa Maria Santos, Andres Stutzin, Harvey B. Pollard
      Pages 163-178
  4. Channels in Biological Membranes

    1. Front Matter
      Pages 179-179
    2. Frederick Sachs
      Pages 181-193
    3. Nava Moran, Gerald Ehrenstein, Kunihiko Iwasa, Charles Bare, Charles Mischke
      Pages 195-205
    4. Sandra Guggino
      Pages 207-220
    5. Juan Bacigalupo
      Pages 221-233
    6. M. Luxoro, V. Nassar-Gentina
      Pages 235-242
    7. Clay M. Armstrong
      Pages 243-254
  5. Ionic Channel Reconstitution

    1. Front Matter
      Pages 255-255
    2. Christopher Miller
      Pages 257-271
    3. Robert J. French, Bruce K. Krueger, Jennings F. Worley III
      Pages 273-290
    4. A. Darszon, J. García-Soto, A. Liévano, J. A. Sánchez, A. D. Islas-Trejo
      Pages 291-305
    5. Daniel Wolff, Cecilia Vergara, Ximena Cecchi, Ramon Latorre
      Pages 307-322
  6. Ionic Channel Modulation

    1. Front Matter
      Pages 323-323
    2. Irwin B. Levitan
      Pages 325-334
    3. Werner R. Loewenstein
      Pages 335-351
    4. Illani Atwater, John Rinzel
      Pages 353-362
  7. Ionic Channel Structure, Functions, and Models

  8. Back Matter
    Pages 431-437

About this book


This book is based on a series of lectures for a course on ionic channels held in Santiago, Chile, on November 17-20, 1984. It is intended as a tutorial guide on the properties, function, modulation, and reconstitution of ionic channels, and it should be accessible to graduate students taking their first steps in this field. In the presentation there has been a deliberate emphasis on the spe­ cific methodologies used toward the understanding of the workings and function of channels. Thus, in the first section, we learn to "read" single­ channel records: how to interpret them in the theoretical frame of kinetic models, which information can be extracted from gating currents in re­ lation to the closing and opening processes, and how ion transport through an open channel can be explained in terms of fluctuating energy barriers. The importance of assessing unequivocally the origin and purity of mem­ brane preparations and the use of membrane vesicles and optical tech­ niques in the stUGY of ionic channels are also discussed in this section. The patch-clamp technique has made it possible to study ion channels in a variety of different cells and tissues not amenable to more conven­ tional electrophysiological methods. The second section, therefore, deals with the use of this technique in the characterization of ionic channels in different types of cells, ranging from plant protoplasts to photoreceptors.


cells fluorescence physiology skeletal muscle tissue

Editors and affiliations

  • Ramon Latorre
    • 1
  1. 1.Centro de Estudios Científicos de Santiago and Facultad de CienciasUniversidad de ChileSantiagoChile

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag US 1986
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4684-5079-8
  • Online ISBN 978-1-4684-5077-4
  • Series Print ISSN 1571-571X
  • Buy this book on publisher's site