Short-Term Bioassays in the Analysis of Complex Environmental Mixtures II

  • Michael D. Waters
  • Shahbeg S. Sandhu
  • Joellen Lewtas Huisingh
  • Larry Claxton
  • Stephen Nesnow

Part of the Environmental Science Research book series (ESRH, volume 22)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Keynote Address

    1. Front Matter
      Pages 1-1
    2. Vilma Hunt
      Pages 3-6
  3. Ambient Air

    1. Front Matter
      Pages 7-7
    2. Joellen Lewtas Huisingh
      Pages 9-19
    3. Alan Kolber, Thomas Wolff, Thomas Hughes, Edo Pellizzari, Charles Sparacino, Michael Waters et al.
      Pages 21-43
    4. R. Jungers, R. Burton, L. Claxton, J. Lewtas Huisingh
      Pages 45-65
    5. C. Peter Flessel, Jerome J. Wesolowski, SuzAnne Twiss, James Cheng, Joel Ondo, Nadine Monto et al.
      Pages 67-83
    6. Ronald E. Rasmussen, T. Timothy Crocker
      Pages 119-131
  4. Drinking Water and Aqueous Effluents

    1. Front Matter
      Pages 133-133
    2. John C. Loper, M. Wilson Tabor
      Pages 155-165
    3. Merrel Robinson, John W. Glass, David Cmehil, Richard J. Bull, John G. Orthoefor
      Pages 177-188
    4. William D. Ross, William J. Hillan, Mark T. Wininger, JoAnne Gridley, Lan Fong Lee, Richard J. Hare et al.
      Pages 189-199
  5. Terrestrial Systems

  6. Mobile Sources

  7. Stationary Sources

    1. Front Matter
      Pages 369-369
    2. R. G. Merrill Jr., W. W. McFee, N. A. Jaworski
      Pages 371-377
    3. Gerald L. Fisher, Clarence E. Chrisp, Floyd D. Wilson
      Pages 379-393
    4. Catherine Aranyi, Jeannie Bradof, Donald E. Gardner, Joellen Lewtas Huisingh
      Pages 431-443
    5. Avram Gold, Eric Eisenstadt, Stephen Nesnow, Martha M. Moore, Helen Garland, Gaynelle Curtis et al.
      Pages 445-460
    6. Rita Schoeny, David Warshawsky, Lois Hollingsworth, Mary Hund, George Moore
      Pages 461-475
  8. Hazard Assessment

  9. Back Matter
    Pages 513-524

About this book


More than one hundred short-term bioassays are now available for detecting the toxicity, mutagenicity, and potential carcinogenicity of chemicals. These bioassays were developed and validated with individual compounds, and their principal application was perceived to be in evaluating the health hazard of such materials. However, man is rarely exposed to single chemicals; his exposure to hazardous chemicals is more commonly a multifactorial phenomenon. Although chemical analysis can be used to detect known hazardous compounds, it would be a staggering and expensive task to analyze large numbers of samples for all known or suspected hazardous constituents. Furthermore, the biological activity of a complex mixture cannot be reliably predicted from knowledge of its components. On the other hand, bioassays alone cannot tell us which components of complex mixtures are responsible for the biological activity detected. Thus, cost effectiveness and technical feasibility dictate stepwise and perhaps iterative application -of both chemical and biological methods in evaluating the health effects of complex environmental mixtures. Through the coupling of reliable biological detection systems with methods of chemical fractionation and analysis, it is frequently possible to isolate the individual chemical species that show biological activity. Initially, complex mixtures may be separated and bioassayed in carefully defined chemical fractions. The results of such short-term screening bioassays then may be used td guide the course of further fractionation and to determine the need for more stringent and comprehensive biological testing.


Potential cancer mutagenesis research toxicity

Editors and affiliations

  • Michael D. Waters
    • 1
  • Shahbeg S. Sandhu
    • 1
  • Joellen Lewtas Huisingh
    • 1
  • Larry Claxton
    • 1
  • Stephen Nesnow
    • 1
  1. 1.U. S. Environmental Protection AgencyResearch Triangle ParkUSA

Bibliographic information