Tissue Culture of the Nervous System

  • Gordon Sato

Part of the Current Topics in Neurobiology book series (CTNB)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. David Schubert, A. John Harris, Stephen Heinemann, Y. Kidokoro, James Patrick, Joseph Henry Steinbach
    Pages 55-86
  3. G. Augusti-Tocco, E. Parisi, F. Zucco, L. Casola, M. Romano
    Pages 87-106
  4. Roger N. Rosenberg
    Pages 107-134
  5. Harvey R. Herschman, Barbara P. Grauling, Michael P. Lerner
    Pages 187-202
  6. S. E. Pfeiffer
    Pages 203-230
  7. Allen C. Stoolmiller, Glyn Dawson, Albert Dorfman, Joseph P. Kennedy Jr.
    Pages 247-280
  8. Back Matter
    Pages 281-288

About this book


The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad­ vantages of these cultures.


biology cell cell biology embryo neurobiology tissue

Editors and affiliations

  • Gordon Sato
    • 1
  1. 1.Department of BiologyUniversity of California, San DiegoLa JollaUSA

Bibliographic information