Methods of Cell Separation

1. Front Matter

   Pages i-xiv

   PDF

2. Preparative Density Gradient Electrophoresis and Velocity Sedimentation at Unit Gravity of Mammalian Cells

   • Nicholas Catsimpoolas, Ann L. Griffith

   Pages 1-24

3. Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloidal Silica Gradients

   • Håkan Pertoft, Torvard C. Laurent

   Pages 25-65

4. Dielectrophoresis: Applications to the Characterization and Separation of Cells

   • Herbert A. Pohl

   Pages 67-169

5. Separation of Viable Cells by Velocity Sedimentation in an Isokinetic Gradient of Ficoll in Tissue Culture Medium

   • Thomas G. Pretlow II, Theresa P. Pretlow

   Pages 171-191

6. Fractionation and Manipulation of Cells with Chemically Modified Fibers and Surfaces

   • Urs S. Rutishauser, Gerald M. Edelman

   Pages 193-228

7. The Separation of Lymphoid Cells on the Basis of Physical Parameters: Separation of B- and T-Cell Subsets and Characterization of B-Cell Differentiation Stages

   • Ken Shortman

   Pages 229-249

8. Multiparameter Analysis and Sorting of Mammalian Cells

   • J. A. Steinkamp

   Pages 251-306

9. Partition of Cells in Two-Polymer Aqueous Phases: A Surface Affinity Method for Cell Separation

   • Harry Walter

   Pages 307-354

10. Back Matter

    Pages 355-361

    PDF

Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu­ nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ­ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, specialization, interactions, cytotoxicity, and transformation. Clinical diag­ nosis of diseased states and use of isolated cells for therapeutic (e. g. , immunotherapy) or survival (e. g. , transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However. the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-end treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures pres­ ently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological prob­ lems. To this end, I was fortunate to obtain the contribution of certain leading scientists in the field of cell separation, people who in their pioneer­ ing work have struggled with the particular problems involved in separating living cells and in some way have won.

• blood cell
• cancer
• cancer research
• cell
• cells
• cognition
• cytotoxicity
• development
• immunotherapy
• instrumentation
• leukocyte
• research
• therapy
• tissue
• toxicity

• Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, USA

  Nicholas Catsimpoolas

Policies and ethics